Crosstalk between vertical and horizontal gene transfer: plasmid replication control by a conjugative relaxase

Nucleic Acids Research
Fabián Lorenzo-DíazM Espinosa

Abstract

Horizontal gene transfer is a key process in the evolution of bacteria and also represents a source of genetic variation in eukaryotes. Among elements participating in gene transfer, thousands of small (<10 kb) mobile bacterial plasmids that replicate by the rolling circle mechanism represent a driving force in the spread of antibiotic resistances. In general, these plasmids are built as genetic modules that encode a replicase, an antibiotic-resistance determinant, and a relaxase that participates in their conjugative mobilization. Further, they control their relatively high copy number (∼30 copies per genome equivalent) by antisense RNAs alone or combined with a repressor protein. We report here that the MobM conjugative relaxase encoded by the promiscuous plasmid pMV158 participates in regulation of the plasmid copy number by transcriptional repression of the antisense RNA, thus increasing the number of plasmid molecules ready to be horizontally transferred (mobilization) and/or vertically inherited (replication). This type of crosstalk between genetic modules involved in vertical and horizontal gene flow has not been reported before.

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Citations

Dec 9, 2017·Frontiers in Microbiology·Stephen M KwongNeville Firth
Dec 19, 2017·Frontiers in Microbiology·Paweł WawrzyniakDariusz Bartosik
Aug 4, 2018·Wiley Interdisciplinary Reviews. RNA·Radoslaw Pluta, Manuel Espinosa
Jan 24, 2019·Journal of Bacteriology·Frances Tran, James Q Boedicker
Mar 31, 2018·Frontiers in Molecular Biosciences·Fabián Lorenzo-DíazManuel Espinosa
Jun 14, 2019·Frontiers in Microbiology·Verena KohlerElisabeth Grohmann
Jan 24, 2021·Proceedings of the National Academy of Sciences of the United States of America·Songwei NiXiaoxue Wang
Feb 4, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Mariam Nasser AljaafariSwee-Hua Erin Lim
Feb 24, 2021·Microbial Genomics·Sergio Mascarenhas Morgado, Ana Carolina Paulo Vicente
May 1, 2021·Microorganisms·Inmaculada Moreno-CórdobaManuel Espinosa
Jul 13, 2021·Frontiers in Molecular Biosciences·Virtu Solano-ColladoAlicia Bravo
Dec 9, 2021·Microbiology and Molecular Biology Reviews : MMBR·M Pilar Garcillán-BarciaManuel Espinosa

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Methods Mentioned

BETA
PCR
footprinting
electrophoresis
electrophoretic mobility shift
Electron
electron microscopy

Software Mentioned

Mfold
SigmaPlot
MobM
Quantity One

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