Crosstalk between Wnt/β-catenin and estrogen receptor signaling synergistically promotes osteogenic differentiation of mesenchymal progenitor cells.

PloS One
Yanhong GaoTong-Chuan He

Abstract

Osteogenic differentiation from mesenchymal progenitor cells (MPCs) are initiated and regulated by a cascade of signaling events. Either Wnt/β-catenin or estrogen signaling pathway has been shown to play an important role in regulating skeletal development and maintaining adult tissue homeostasis. Here, we investigate the potential crosstalk and synergy of these two signaling pathways in regulating osteogenic differentiation of MPCs. We find that the activation of estrogen receptor (ER) signaling by estradiol (E2) or exogenously expressed ERα in MPCs synergistically enhances Wnt3A-induced early and late osteogenic markers, as well as matrix mineralization. The E2 or ERα-mediated synergy can be effectively blocked by ERα antagonist tamoxifen. E2 stimulation can enhance endochondral ossification of Wnt3A-transduced mouse fetal limb explants. Furthermore, exogenously expressed ERα significantly enhances the maturity and mineralization of Wnt3A-induced subcutaneous and intramuscular ectopic bone formation. Mechanistically, we demonstrate that E2 does not exert any detectable effect on β-catenin/Tcf reporter activity. However, ERα expression is up-regulated within the first 48h in AdWnt3A-transduced MPCs, whereas ERβ expression is s...Continue Reading

References

Apr 16, 1998·Proceedings of the National Academy of Sciences of the United States of America·T C HeB Vogelstein
Sep 4, 1998·Science·T C HeK W Kinzler
Apr 1, 1999·Menopause : the Journal of the North American Menopause Society·F Kronenberg, C Hughes
Apr 2, 1999·Science·M F PittengerD R Marshak
Dec 22, 1999·The Journal of Clinical Endocrinology and Metabolism·M M Grumbach, R J Auchus
Oct 14, 2000·Annual Review of Cell and Developmental Biology·B R OlsenW Wang
Nov 4, 2000·Bone·K KusuzakiY Hirasawa
Jun 8, 2001·Human Reproduction Update·A Juul
Aug 20, 2003·The Journal of Bone and Joint Surgery. American Volume·Hongwei ChengTong-Chuan He
Nov 25, 2003·Journal of Cellular Biochemistry·Ying PengTong-Chuan He
Mar 3, 2004·Molecular Interventions·Jason Matthews, Jan-Ake Gustafsson
Aug 12, 2004·The Journal of Biological Chemistry·Alexander P KouzmenkoShigeaki Kato
Feb 8, 2005·Molecular Endocrinology·Linda Björnström, Maria Sjöberg
Feb 10, 2007·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Hue H LuuTong-Chuan He
May 25, 2007·Current Osteoporosis Reports·Roland Baron, Georges Rawadi
Sep 22, 2007·Journal of Cellular Physiology·Boris J CheskisLeonard P Freedman
Nov 6, 2007·Frontiers in Bioscience : a Journal and Virtual Library·Zhong-Liang DengTong-Chuan He
Mar 8, 2008·Clinical Pharmacology and Therapeutics·J J Schrogie
Oct 8, 2008·Laboratory Investigation; a Journal of Technical Methods and Pathology·Xiaoji LuoTong-Chuan He
Dec 17, 2008·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Bart O Williams, Karl L Insogna
Jun 30, 2009·Differentiation; Research in Biological Diversity·Gao-Hui ZhuTong-Chuan He
Sep 10, 2009·Liver International : Official Journal of the International Association for the Study of the Liver·Jiayi HuangNi Tang
Mar 17, 2010·Biochemical and Biophysical Research Communications·Astrid LiedertAnita Ignatius
Apr 8, 2010·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Bai-Cheng HeRex C Haydon
May 26, 2010·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Liang ChenZhong-Liang Deng
Sep 10, 2010·Current Molecular Pharmacology·Eric R WagnerTong-Chuan He

❮ Previous
Next ❯

Citations

Jan 24, 2016·The Journal of Steroid Biochemistry and Molecular Biology·Zhixiang XuXuejun Pan
Dec 3, 2015·Proceedings of the National Academy of Sciences of the United States of America·Sofia Movérare-SkrticClaes Ohlsson
Mar 20, 2016·Environmental Toxicology and Pharmacology·Alexander G KramerChristopher S Lassiter
Jun 16, 2015·Environment International·Ryoiti Kiyama, Yuko Wada-Kiyama
Apr 22, 2014·Aquatic Toxicology·Sarah P CohenChristopher S Lassiter
Jun 17, 2016·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Junfeng LiZhaowen Zong
Mar 27, 2018·Toxicology and Industrial Health·Benjamin S WalkerChristopher S Lassiter
Feb 28, 2018·Journal of Medicinal Food·Jeremy E Davis, Darcie Hastings
Dec 5, 2017·Phytochemistry Reviews : Proceedings of the Phytochemical Society of Europe·Elisa Torre
Dec 19, 2014·Endocrinology·Jon E WergedalSubburaman Mohan
Mar 9, 2018·Expert Reviews in Molecular Medicine·Chithra Janardhanan SusheelammaSivakumari Asha Nair
Jun 12, 2018·Frontiers in Neuroscience·Matthew C S DenleyDeepak P Srivastava
Sep 20, 2020·Biology of Reproduction·Rong LiFrancesco J DeMayo
Oct 18, 2019·Frontiers in Endocrinology·Bin ZhangJordan D Miller
Nov 9, 2019·International Journal of Molecular Sciences·Kazuhiro MaedaKeishi Marumo
Feb 6, 2020·Journal of the American Heart Association·Allison L KuipersJoseph M Zmuda

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
Assay
dissection
fluorescence microscopy

Software Mentioned

Primer3
Amira

Related Concepts

Related Feeds

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.

Calcium & Bioenergetics

Bioenergetic processes, including cellular respiration and photosynthesis, concern the transformation of energy by cells. Here is the latest research on the role of calcium in bioenergetics.

Cardiomyopathy

Cardiomyopathy is a disease of the heart muscle, that can lead to muscular or electrical dysfunction of the heart. It is often an irreversible disease that is associated with a poor prognosis. There are different causes and classifications of cardiomyopathies. Here are the latest discoveries pertaining to this disease.