Crosstalk between Wnt signaling and Phorbol ester-mediated PKC signaling in MCF-7 human breast cancer cells

Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie
Soyoung KimKyung-Soo Nam

Abstract

Although many studies have implicated the crosstalk between the Wnt and PKC signaling pathways in tumor initiation and progression, the molecular roles of PKC isoforms in the Wnt signaling pathway remain poorly understood. In this study, we explored the contribution of PKC isoforms to canonical and noncanonical Wnt signaling pathway in mediating cell migration and an epithelial-mesenchymal transition (EMT). When MCF-7 cells were treated with 12-O-tetradecanoylphorbol-13-acetate (TPA) for up to 3 weeks, the effect of TPA on Wnt signaling pathway was dramatically different depending on the exposure time. The short term exposure (3 days) of MCF-7 cells to TPA exhibited significant induction of Wnt5a expression, along with the enhanced expression of PKC-α, to promote cell migration, which suggested that activation of noncanonical Wnt signaling pathway is associated with PKC-α. However, the chronic exposure (3 weeks) of cells to TPA completely suppressed Wnt5a expression and the expression of PKC-η and PKC-δ, whereas the expression of Wnt3a and PKC-θ were up-regulated to activate the canonical Wnt signaling pathway. Moreover, the loss of epithelial markers, including E-cadherin and GATA-3, suggested that chronic exposure of TPA stim...Continue Reading

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Citations

Jun 9, 2017·Cell Cycle·Azhar JabareenMahmoud Huleihel
Dec 23, 2016·Medical Science Monitor : International Medical Journal of Experimental and Clinical Research·Ruolan ZengZhifu Wu
Jul 25, 2018·Proceedings of the National Academy of Sciences of the United States of America·Zijie SuDesheng Lu
May 29, 2021·Breast Cancer : the Journal of the Japanese Breast Cancer Society·Ai AmiokaMorihito Okada

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