PMID: 8599180Oct 1, 1995Paper

Crotavirin, a potent platelet aggregation inhibitor purified from the venom of the snake Crotalus viridis

Toxicon : Official Journal of the International Society on Toxinology
C Z LiuT F Huang

Abstract

A potent platelet aggregation inhibitor in the venom of Crotalus viridis snake was purified to homogeneity by gel filtration chromatography and reverse phase high-performance liquid chromatography. This purified principle, named crotavirin, is a single-chain polypeptide with a mol. wt of 9200 as estimated by SDS-polyacrylamide gel electrophoresis. It inhibited the aggregation of human washed platelets induced by collagen, thrombin and thomboxane analogue (U46619) with a similar IC50 (approximately 1.0 micrograms/ml, 0.11 microM). The binding of fluorescein isothiocyanate-conjugated crotavirin to platelets was abolished in the presence of divalent cation chelator, EDTA, indicating that divalent cation is essential for crotavirin's binding. A monoclonal antibody, 7E3, raised against platelet glycoprotein IIb-IIIa complex blocked the binding of fluorescein isothiocyanate-conjugated crotavirin to platelets, whereas the other monoclonal antibody against glycoprotein IIb-IIIa, 10E5, had no inhibitory effect. In addition, crotavirin inhibited in a concentration-dependent manner the binding of fluorescein isothiocyanate-conjugated rhodostomin, a member of the disintegrin family, to platelets. Its binding to platelets was blocked by dis...Continue Reading

References

Jul 15, 1991·The Biochemical Journal·T F HuangC M Teng
May 24, 1991·Biochimica Et Biophysica Acta·T F HuangC Ouyang
Nov 1, 1990·Proceedings of the Society for Experimental Biology and Medicine·R J GouldS Niewiarowski
Jan 1, 1986·Toxicon : Official Journal of the International Society on Toxinology·C H OuyangT F Huang
Feb 1, 1972·British Journal of Haematology·J F MustardM A Packham
Oct 1, 1974·British Journal of Haematology·A T Nurden, J P Caen
Jun 1, 1972·Proceedings of the Society for Experimental Biology and Medicine·W J Dodds, R J Pickering
Mar 29, 1994·Proceedings of the National Academy of Sciences of the United States of America·G Weskamp, C P Blobel
Aug 1, 1963·The Journal of Physiology·G V BORN, M J CROSS

❮ Previous
Next ❯

Citations

May 9, 2007·Journal of Thrombosis and Thrombolysis·Juan C López-JohnstonAlexis Rodríguez-Acosta
Mar 30, 2010·Toxicon : Official Journal of the International Society on Toxinology·Ying-Ming WangInn-Ho Tsai
Sep 27, 2003·Biochemical and Biophysical Research Communications·Run-Qiang ChenYu-Liang Xiong
Jun 1, 2005·Toxicon : Official Journal of the International Society on Toxinology·Neville Marsh, Vaughan Williams
Oct 27, 2018·Current Drug Targets·Pedro Henrique Souza CesarSilvana Marcussi
Apr 4, 2021·International Journal of Molecular Sciences·Danique L van den KerkhofIngrid Dijkgraaf

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.