CRP2, a new invadopodia actin bundling factor critically promotes breast cancer cell invasion and metastasis

Oncotarget
Céline HoffmannClément Thomas

Abstract

A critical process underlying cancer metastasis is the acquisition by tumor cells of an invasive phenotype. At the subcellular level, invasion is facilitated by actin-rich protrusions termed invadopodia, which direct extracellular matrix (ECM) degradation. Here, we report the identification of a new cytoskeletal component of breast cancer cell invadopodia, namely cysteine-rich protein 2 (CRP2). We found that CRP2 was not or only weakly expressed in epithelial breast cancer cells whereas it was up-regulated in mesenchymal/invasive breast cancer cells. In addition, high expression of the CRP2 encoding gene CSRP2 was associated with significantly increased risk of metastasis in basal-like breast cancer patients. CRP2 knockdown significantly reduced the invasive potential of aggressive breast cancer cells, whereas it did not impair 2D cell migration. In keeping with this, CRP2-depleted breast cancer cells exhibited a reduced capacity to promote ECM degradation, and to secrete and express MMP-9, a matrix metalloproteinase repeatedly associated with cancer progression and metastasis. In turn, ectopic expression of CRP2 in weakly invasive cells was sufficient to stimulate cell invasion. Both GFP-fused and endogenous CRP2 localized to ...Continue Reading

References

Dec 2, 1992·International Journal of Cancer. Journal International Du Cancer·A ZyadS Chouaib
Aug 1, 1997·Molecular and Cellular Biology·Y KongS F Konieczny
Mar 30, 2002·Science·Lisa M CoussensLynn M Matrisian
Jan 18, 2003·Developmental Cell·David F ChangRobert J Schwartz
Jan 23, 2003·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Ralf Weiskirchen, Kalle Günther
Feb 17, 2005·Journal of Cell Science·Joo-ri Kim-KaneyamaMotoko Shibanuma
Mar 23, 2005·Molecular Genetics and Metabolism·Bill NewmanKatherine A Siminovitch
Dec 13, 2005·BMC Cell Biology·Thuan C TranJeffrey A Greenwood
Aug 15, 2006·The Plant Cell·Clément ThomasAndré Steinmetz
May 29, 2008·Human Molecular Genetics·Christian GeierCemil Ozcelik
Mar 17, 2009·Biochemical and Biophysical Research Communications·Hyo Sang Jang, Jeffrey A Greenwood
Apr 11, 2009·Oncology Reports·M W RoomiA Niedzwiecki
Sep 19, 2009·Methods in Molecular Biology·Dennis BreitsprecherJan Faix
Jan 2, 2010·Circulation Research·Ralph KnöllKenneth R Chien
Apr 16, 2010·Nature·Lin ChenXin-Yun Huang
Apr 28, 2010·The Journal of Cell Biology·Marie SchoumacherDanijela M Vignjevic
Aug 18, 2010·Communicative & Integrative Biology·Laura M Machesky, Ang Li
Dec 22, 2010·Nature Cell Biology·Kristopher E Kubow, Alan Rick Horwitz
Jun 21, 2011·The Journal of Biological Chemistry·Silvia JansenRoberto Dominguez
Jun 24, 2011·Nature Reviews. Molecular Cell Biology·Danielle A Murphy, Sara A Courtneidge
Mar 6, 2012·Journal of Cell Science·Bojana GligorijevicJohn Condeelis
Apr 25, 2012·The Journal of Cell Biology·Jeanine PignatelliChristopher E Turner
Jun 23, 2012·European Journal of Cell Biology·Pasquale CerveroStefan Linder
Nov 28, 2012·The Journal of Biological Chemistry·Shengyu YangXin-Yun Huang
Nov 10, 2013·Nature Medicine·Liling WanYibin Kang
Nov 29, 2013·Journal of Cell Science·Céline HoffmannClément Thomas

❮ Previous
Next ❯

Citations

Aug 17, 2017·PloS One·Takanori KiharaJun Miyake
Oct 11, 2017·Planta·Céline HoffmannClément Thomas
Aug 28, 2020·Frontiers in Physiology·Xiaolan HuangJingxing Dai
Feb 26, 2019·Frontiers in Immunology·Maria PapadakiEleni Douni
Aug 30, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Hamad Ali HamadNur Fariesha Md Hashim
Sep 27, 2020·Molecular Biology Reports·Marine GeoffroyIsabelle Grillier-Vuissoz
Jan 8, 2021·International Journal of Molecular Sciences·Luca BiniLaura Bianchi
Dec 23, 2020·Scientific Reports·Takumi HigakiKaoru Katoh
Feb 24, 2021·Proceedings of the National Academy of Sciences of the United States of America·Simone M BrändleKlaus Dornmair
Aug 10, 2021·American Journal of Respiratory Cell and Molecular Biology·Ankit HanmandluHarry Karmouty-Quintana

❮ Previous
Next ❯

Datasets Mentioned

BETA
BC000992

Methods Mentioned

BETA
PMA
fluorescence microscopy
xenograft
PCR
electrophoresis
Assay
bioluminescence imaging

Software Mentioned

WoundMaker
ImageJ
Huygens essential Software
Filter2d ThinLine
Pantomics
MTrackJ
Kbi

Related Concepts

Related Feeds

Cell Migration in Cancer and Metastasis

Migration of cancer cells into surrounding tissue and the vasculature is an initial step in tumor metastasis. Discover the latest research on cell migration in cancer and metastasis here.

Breast Invasive Carcinoma (Keystone)

Invasive breast cancers indicate a spread into breast tissues and lymph nodes. Here are the latest discoveries pertaining to breast invasive carcinomas.

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.

Breast Cancer Triple-N

Breast cancer cells have receptors for estrogen, progesterone, HER2 receptors (also called ERBB2). Triple-negative breast cancers do not have any of these receptors. Here are the latest discoveries pertaining to triple-negative breast cancers.

Breast Invasive Carcinoma

Invasive breast cancers indicate a spread into breast tissues and lymph nodes. Here are the latest discoveries pertaining to breast invasive carcinomas.