Crucifera sulforaphane (SFN) inhibits the growth of nasopharyngeal carcinoma through DNA methyltransferase 1 (DNMT1)/Wnt inhibitory factor 1 (WIF1) axis.

Phytomedicine : International Journal of Phytotherapy and Phytopharmacology
Luo ChenNai Ki Mak

Abstract

Sulforaphane (SFN), a natural compound present in cruciferous vegetable, has been shown to possess anti-cancer activities. Cancer stem cell (CSC) in bulk tumor is generally considered as treatment resistant cell and involved in cancer recurrence. The effects of SFN on nasopharyngeal carcinoma (NPC) CSCs have not yet been explored. The present study aims to examine the anti-tumor activities of SFN on NPC cells with CSC-like properties and the underlying mechanisms. NPC cells growing in monolayer culture, CSCs-enriched NPC tumor spheres, and also the NPC nude mice xenograft were used to study the anti-tumor activities of SFN on NPC. The population of cells expressing CSC-associated markers was evaluated using flow cytometry and aldehyde dehydrogenase (ALDH) activity assay. The effect of DNA methyltransferase 1 (DNMT1) on the growth of NPC cells was analyzed by using small interfering RNA (siRNA)-mediated silencing method. SFN was found to inhibit the formation of CSC-enriched NPC tumor spheres and reduce the population of cells with CSC-associated properties (SRY (Sex determining Region Y)-box 2 (SOX2) and ALDH). In the functional study, SFN was found to restore the expression of Wnt inhibitory factor 1 (WIF1) and the effect was ...Continue Reading

Citations

Sep 10, 2020·Frontiers in Pharmacology·Sergi Herve AkoneRolf Müller
May 20, 2020·Frontiers in Pharmacology·Cinzia CalcabriniCarmela Fimognari
Jan 13, 2021·Seminars in Cancer Biology·Madhumitha Kedhari SundaramArif Hussain
Nov 19, 2019·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Parham Jabbarzadeh KaboliZhangang Xiao
Jul 18, 2021·Seminars in Cancer Biology·Man ChuZhi-Wei Wang

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