Crumbs 2 prevents cortical abnormalities in mouse dorsal telencephalon

Neuroscience Research
Jacobus J DudokJan Wijnholds

Abstract

The formation of a functionally integrated nervous system is dependent on a highly organized sequence of events that includes timely division and differentiation of progenitors. Several apical polarity proteins have been shown to play crucial roles during neurogenesis, however, the role of Crumbs 2 (CRB2) in cortical development has not previously been reported. Here, we show that conditional ablation of Crb2 in the murine dorsal telencephalon leads to defects in the maintenance of the apical complex. Furthermore, within the mutant dorsal telencephalon there is premature expression of differentiation proteins. We examined the physiological function of Crb2 on wild type genetic background as well as on background lacking Crb1. Telencephalon lacking CRB2 resulted in reduced levels of PALS1 and CRB3 from the apical complex, an increased number of mitotic cells and expanded neuronal domain. These defects are transient and therefore only result in rather mild cortical abnormalities. We show that CRB2 is required for maintenance of the apical polarity complex during development of the cortex and regulation of cell division, and that loss of CRB2 results in cortical abnormalities.

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Citations

Mar 24, 2018·Journal of Neurochemistry·Ana UzquianoFiona Francis
Mar 10, 2020·PLoS Biology·Christine M TaitMarysia Placzek
May 10, 2017·Neural Development·Jennifer RakotomamonjyAnjen Chenn
Jun 20, 2019·Frontiers in Cellular Neuroscience·Janne HakanenFadel Tissir
Sep 15, 2020·Frontiers in Neuroscience·Nanda BoonLucie P Pellissier
Oct 20, 2020·Human Molecular Genetics·Noelle SterlingPankaj B Agrawal
Dec 18, 2020·The Journal of Cell Biology·Masataka KuniiAkihiro Harada
Mar 11, 2021·Journal of the American Society of Nephrology : JASN·Annika Möller-KeruttThomas Weide
Jun 3, 2020·Molecular and Cellular Neurosciences·Yves Jossin
Jul 30, 2021·Frontiers in Molecular Neuroscience·Francesco Bedogni, Robert F Hevner

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