Crustacean hyperglycaemic hormone (CHH)-like peptides and CHH-precursor-related peptides from pericardial organ neurosecretory cells in the shore crab, Carcinus maenas, are putatively spliced and modified products of multiple genes

The Biochemical Journal
H DircksenS G Webster

Abstract

About 24 intrinsic neurosecretory neurons within the pericardial organs (POs) of the crab Carcinus maenas produce a novel crustacean hyperglycaemic hormone (CHH)-like peptide (PO-CHH) and two CHH-precursor-related peptides (PO-CPRP I and II) as identified immunochemically and by peptide chemistry. Edman sequencing and MS revealed PO-CHH as a 73 amino acid peptide (8630 Da) with a free C-terminus. PO-CHH and sinus gland CHH (SG-CHH) share an identical N-terminal sequence, positions 1-40, but the remaining sequence, positions 41-73 or 41-72, differs considerably. PO-CHH may have different precursors, as cDNA cloning of PO-derived mRNAs has revealed several similar forms, one exactly encoding the peptide. All PO-CHH cDNAs contain a nucleotide stretch coding for the SG-CHH(41-76) sequence in the 3'-untranslated region (UTR). Cloning of crab testis genomic DNA revealed at least four CHH genes, the structure of which suggest that PO-CHH and SG-CHH arise by alternative splicing of precursors and possibly post-transcriptional modification of PO-CHH. The genes encode four exons, separated by three variable introns, encoding part of a signal peptide (exon I), the remaining signal peptide residues, a CPRP, the PO-CHH(1-40)/SG-CHH(1-40) se...Continue Reading

References

Jun 22, 1991·Proceedings. Biological Sciences·S G Webster
Nov 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·W J SchielenW Nieuwenhuizen
Oct 14, 1967·Nature·M StefaniniL Zamboni
Dec 1, 1995·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·D P De Kleijn, F Van Herp
Mar 17, 1999·Archives of Insect Biochemistry and Physiology·A MacinsJ E Phillips
Nov 11, 1999·Proceedings of the National Academy of Sciences of the United States of America·J S ChungS G Webster
Apr 4, 2000·Insect Biochemistry and Molecular Biology·H EndoT Watanabe
Oct 6, 2000·General and Comparative Endocrinology·C Spanings-PierrotG Charmantier
Sep 1, 1961·General and Comparative Endocrinology·D M MAYNARD

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Citations

Jun 4, 2005·Marine Biotechnology·S H ChenC M Kuo
Nov 26, 2003·General and Comparative Endocrinology·Siu-Ming ChanStephen S Tobe
Jun 5, 2003·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Cedomil Lucu, David W Towle
Feb 27, 2010·BMC Evolutionary Biology·Nicolas MontagnéJean-Yves Toullec
Aug 21, 2010·Cellular and Molecular Life Sciences : CMLS·Andrew E ChristiePatsy S Dickinson
Jul 31, 2014·Journal of Experimental Zoology. Part A, Ecological Genetics and Physiology·Ch SwethaP Sreenivasula Reddy
Dec 7, 2010·Integrative Biology : Quantitative Biosciences From Nano to Macro·G Purna Chandra NagarajuD W Borst
Dec 23, 2009·General and Comparative Endocrinology·J Sook ChungN Tsutsui

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