Crustacean larval factor shares structural characteristics with the insect-specific follicle cell protein

Scientific Reports
Tomer VenturaAbigail Elizur

Abstract

Literature on the cuticle formation in larval stages of the diverse group of decapod crustaceans is lacking, as opposed to a wealth of knowledge in several insect groups. Here we provide the first glimpse of the cuticular organisation in larvae of the eastern spiny lobster Sagmariasus verreauxi. A bioinformatic approach applied to S. verreauxi transcriptome through metamorphosis identified for the first time a small secreted protein with multiple isoforms that is highly expressed in crustacean larvae. This protein, named crustacean larval factor (Clf) shares structural characteristics with insect follicle cell protein 3 (FCP3), an insect-specific, rapidly evolving protein, with spatial-temporal regulated expression that is restricted to follicular cells during the production of the vitellin coat. Furthermore, we identified the FCP3 domain in additional structural proteins in multiple arthropod groups. Recombinant Clf inhibited in vitro calcium carbonate crystalline precipitation, in keeping with the finding that the spiny lobster larval cuticle is mainly composed of amorphous calcium carbonate. In addition, the recombinant Clf was shown to bind chitosan. Taken together, this research identifies two novel structural domains with...Continue Reading

References

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Feb 26, 2016·Scientific Reports·Shmuel BentovAmir Sagi
Apr 22, 2017·The Journal of Steroid Biochemistry and Molecular Biology·Tomer VenturaAbigail Elizur

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Datasets Mentioned

BETA
PRJEB5112

Methods Mentioned

BETA
scanning electron microscopy
PCR
glycosylation

Software Mentioned

TASSER
BLASTp
OrfPredictor
SignalP
TM
MEGA6
tBLASTn
BLAST
ClustalW
noTM

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