Cryo-Electron Microscopy Structure of the Macrobrachium rosenbergii Nodavirus Capsid at 7 Angstroms Resolution

Scientific Reports
Kok Lian HoD Bhella

Abstract

White tail disease in the giant freshwater prawn Macrobrachium rosenbergii causes significant economic losses in shrimp farms and hatcheries and poses a threat to food-security in many developing countries. Outbreaks of Macrobrachium rosenbergii nodavirus (MrNV), the causative agent of white tail disease (WTD) are associated with up to 100% mortality rates. There are no interventions available to treat or prevent MrNV disease however. Here we show the structure of MrNV virus-like particles (VLPs) produced by recombinant expression of the capsid protein, using cryogenic electron microscopy. Our data show that MrNV VLPs package nucleic acids in a manner reminiscent of other known nodavirus structures. The structure of the capsid however shows striking differences from insect and fish infecting nodaviruses, which have been shown to assemble trimer-clustered T = 3 icosahedral virus particles. MrNV particles have pronounced dimeric blade-shaped spikes extending up to 6 nm from the outer surface of the capsid shell. Our structural analysis supports the assertion that MrNV may belong to a new genus of the Nodaviridae. Moreover, our study provides the first structural view of an important pathogen affecting aquaculture industries acros...Continue Reading

References

Mar 1, 1984·Nature·M AdrianA W McDowall
Jan 14, 1994·Journal of Molecular Biology·J P WeryJ E Johnson
Feb 7, 1994·FEBS Letters· Morgunova EYuB K Vainshtein
Jan 1, 1996·Journal of Structural Biology·J R KremerJ R McIntosh
Dec 22, 1999·Journal of Structural Biology·S J LudtkeW Chiu
Jan 3, 2001·Nature Structural Biology·L TangJ E Johnson
Jul 21, 2004·Journal of Computational Chemistry·Eric F PettersenThomas E Ferrin
Aug 10, 2004·Acta Crystallographica. Section D, Biological Crystallography·A ZlotnickJ E Johnson
Nov 23, 2005·Bioinformatics·Konstantin ArnoldTorsten Schwede
May 18, 2006·Annual Review of Microbiology·Anette Schneemann
May 27, 2006·Diseases of Aquatic Organisms·Kalidoss YoganandhanChalor Limsuwan
Mar 1, 2011·Journal of Molecular Biology·E C DykemanR Twarock
May 4, 2011·Journal of Virological Methods·Zee Hong GohWen Siang Tan
Sep 25, 2012·Journal of Structural Biology·Sjors H W Scheres
Jan 16, 2013·Virus Research·Singaiah NaveenKumarIndrani Karunasagar
Jun 1, 2014·The Journal of General Virology·Zee Hong GohWen Siang Tan
Aug 20, 2014·Proceedings of the National Academy of Sciences of the United States of America·Yusong R GuoYizhi J Tao
Apr 22, 2015·International Journal of Nanomedicine·Chean Yeah YongWen Siang Tan
May 8, 2015·Nature Protocols·Lawrence A KelleyMichael J E Sternberg
Nov 26, 2015·Journal of Structural Biology·Kai Zhang
Dec 15, 2015·Nature Communications·Emma L HeskethNeil A Ranson
Oct 26, 2016·SpringerPlus·Murwantoko MurwantokoMasashi Kawaichi

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Citations

Dec 22, 2017·Journal of Virology·Mike StraussMihnea Bostina
Jul 26, 2018·The Journal of General Virology·Qiu Xian ThongWen Siang Tan
Oct 3, 2018·Journal of Fish Diseases·Chen-Fei LowNur Farahiyah Ahmad Nadzri
Mar 10, 2019·Scientific Reports·Qiu Xian ThongWen Siang Tan
Oct 4, 2017·PeerJ·Chean Yeah YongWen Siang Tan
Jul 3, 2021·International Journal of Molecular Sciences·Chun-Hsiung WangWei-Hau Chang
Aug 28, 2021·International Journal of Molecular Sciences·Bethilda Anne SelvarajWen Siang Tan

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Datasets Mentioned

BETA
EMD-3655

Methods Mentioned

BETA
transmission electron microscopy
X-ray
electrophoresis
electron microscopy

Software Mentioned

MODEL
MotionCorr
IMOD
GCTF
Phyre2
EMAN
Relion
SWISS
UCSF Chimera

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