Cryo-electron microscopy studies of human TFIID: conformational breathing in the integration of gene regulatory cues

Structure
Patricia GrobEva Nogales

Abstract

The multisubunit transcription factor TFIID is essential for directing eukaryotic promoter recognition and mediating interactions with activators/cofactors during assembly of the preinitiation complex. Despite its central role in transcription initiation and regulation, structural knowledge of the TFIID complex has so far been largely limited to electron microscopy studies of negatively stained samples. Here, we present a cryo-electron microscopy 3D reconstruction of the large endogenous human TFIID complex. The improved cryopreservation has allowed for a more detailed definition of the structural elements in the complex and for the detection, by an extensive statistical analysis of the data, of a conformational opening and closing of the cavity central to the TFIID architecture. We propose that these density rearrangements in the structure are a likely reflection of the plasticity of the interactions between TFIID and its many partner proteins.

References

Jan 1, 1987·Ultramicroscopy·M UnserA C Steven
Aug 1, 1982·Journal of Microscopy·W O Saxton, W Baumeister
Jun 1, 1994·Current Opinion in Cell Biology·J A Goodrich, R Tjian
Mar 28, 1996·Nature·A HoffmannR G Roeder
May 9, 1996·Nature·S TanT J Richmond
May 10, 1996·Science·J H GeigerP B Sigler
Jan 1, 1996·Journal of Structural Biology·M van HeelM Schatz
Sep 1, 1996·Trends in Biochemical Sciences·R G Roeder
Nov 1, 1996·Genes & Development·G OrphanidesD Reinberg
Dec 22, 1999·Journal of Structural Biology·S J LudtkeW Chiu
Jan 5, 2000·The EMBO Journal·F T Tsai, P B Sigler
Jul 3, 2002·The EMBO Journal·Claire LeurentPatrick Schultz
Jul 26, 2002·Molecular and Cellular Biology·Steven L SandersP Anthony Weil
Oct 5, 2002·Nucleic Acids Research·Tianyuan Zhou, Cheng-Ming Chiang
Apr 22, 2004·Molecular Cell·Antigone KouskoutiIannis Talianidis
May 15, 2004·Molecular and Cellular Biology·Chika SawaStephen Buratowski
Jun 15, 2004·Nature Structural & Molecular Biology·Dylan J TaatjesRobert Tjian
Jul 21, 2004·Journal of Computational Chemistry·Eric F PettersenThomas E Ferrin
Sep 29, 2004·Journal of Structural Biology·J Bernard HeymannAlasdair C Steven
Apr 27, 2005·Cell·Sarah J TilleyHelen R Saibil
Mar 3, 2006·Journal of Structural Biology·Pawel A PenczekChristian M T Spahn

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Citations

Mar 29, 2007·Proceedings of the National Academy of Sciences of the United States of America·Robert J C GilbertJohn E G McCarthy
Jan 18, 2007·Proceedings of the National Academy of Sciences of the United States of America·Suparna BhattacharyaRaymond H Jacobson
Nov 1, 2006·Molecular and Cellular Biology·Krassimira A GarbettP Anthony Weil
Nov 1, 2008·PloS One·Ziv FrankensteinMiriam Eisenstein
Jul 3, 2009·Genes & Development·Wei-Li LiuRobert Tjian
Apr 7, 2011·PLoS Biology·Carrie BerneckyDylan J Taatjes
Sep 21, 2013·Annual Review of Genetics·Hojoong Kwak, John T Lis
Mar 25, 2009·Cellular and Molecular Life Sciences : CMLS·Emilie ClerIrwin Davidson
Jan 5, 2007·Annual Review of Biophysics and Biomolecular Structure·Andres E Leschziner, Eva Nogales
Oct 4, 2011·Methods : a Companion to Methods in Enzymology·Lauren S FisherBridget Carragher
Mar 23, 2011·Current Opinion in Genetics & Development·Gabor PapaiPatrick Schultz
Aug 31, 2010·Biochimica Et Biophysica Acta·Sukesh R Bhaumik
Mar 28, 2007·Current Opinion in Structural Biology·Oscar Llorca
Apr 14, 2016·Journal of Molecular Biology·Kapil GuptaImre Berger
Nov 14, 2012·Biochimie·Maja MalkowskaLucjan Wyrwicz
Oct 1, 2014·Journal of Structural Biology·Carlos Oscar S SorzanoSlavica Jonić
Oct 4, 2013·Critical Reviews in Biochemistry and Molecular Biology·Zachary C PossDylan J Taatjes
Nov 17, 2009·Structure·Francisco J Asturias
Feb 25, 2009·Journal of Structural Biology·Sjors H W ScheresJosé-María Carazo
Jan 22, 2008·Molecular Cell·Wei-Li LiuRobert Tjian
Jan 22, 2013·Cell·Michael A CianfroccoEva Nogales
Jul 11, 2008·Journal of Molecular Biology·Mazdak RadjainiaAlok K Mitra
Feb 19, 2015·Nature Reviews. Molecular Cell Biology·Sarah SainsburyPatrick Cramer
Apr 28, 2016·Biophysical Journal·Carlos Oscar Sanchez SorzanoSlavica Jonić
Oct 11, 2016·Angewandte Chemie·Merle Hantsche, Patrick Cramer
Dec 10, 2016·Transcription·Eva NogalesRobert K Louder
Jun 19, 2017·Current Opinion in Structural Biology·Eva NogalesRobert K Louder
Jul 11, 2006·Nature Structural & Molecular Biology·Megan G ClareyMichael Botchan
Apr 19, 2017·Molecular and Cellular Biology·R A ColemanW L Liu
Mar 2, 2010·The Journal of Biological Chemistry·Justin H LayerP Anthony Weil
Jan 8, 2013·Nature·Christoph BieniossekImre Berger
Mar 8, 2011·Nature Structural & Molecular Biology·Alessandro CostaJames M Berger
May 24, 2017·Annual Review of Biophysics·Eva NogalesYuan He
Nov 9, 2018·Nature Communications·Olga KolesnikovaGabor Papai

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