Cryo-injury and biopreservation

Annals of the New York Academy of Sciences
Alex Fowler, Mehmet Toner

Abstract

Mammalian cells appear to be naturally tolerant to cold temperatures, but the formation of ice when cells are cooled leads to a variety of damaging effects. The study of cryo-injury, therefore, becomes the study of when and how ice is formed both inside and outside the cell during cooling. Protectant chemicals are used to control or prevent ice formation in many preservation protocols, but these chemical themselves tend to be damaging. Cooling and warming rates also strongly affect the amount and location of ice that is formed. Through careful modification of these parameters successful cold preservation techniques for many cell types have been developed, but there are many more cell types that have defied preservation techniques, and the extension of cell-based techniques to tissues and whole organs has been very limited. There are many aspects to the damaging effects of ice in cells that are still poorly understood. In this brief article we review our current understanding of cellular injury and highlight the aspects of cellular injury during cryopreservation that are still poorly understood.

References

Mar 29, 1977·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·J Farrant
Oct 1, 1991·Burns : Journal of the International Society for Burn Injuries·J N Kearney
Feb 1, 1988·Cryobiology·C A KnightA L DeVries
Oct 1, 1971·Cryobiology·S P Leibo, P Mazur
Oct 13, 1965·Annals of the New York Academy of Sciences·P Mazur
Sep 1, 1984·The American Journal of Physiology·P Mazur
Jul 31, 1980·Nature·W F RallJ Farrant
May 14, 1993·Science·R Langer, J P Vacanti
May 1, 1996·Biology of Reproduction·A MartinoS P Leibo
Apr 29, 1998·Annual Review of Physiology·J H CroweL M Crowe
Jan 26, 1999·Annals of the New York Academy of Sciences·A J Fowler, M Toner
Jul 22, 1999·Cryobiology·J P AckerL E McGann
Dec 22, 1999·Human Reproduction·L KuleshovaA Trounson
Mar 4, 2000·Nature Biotechnology·Y C SongM J Taylor
Jan 10, 2002·Fertility and Sterility·Ali ErogluThomas L Toth
Feb 9, 2002·Science·Jocelyn Kaiser
Feb 25, 2003·Chemistry and Physics of Lipids·John H CroweJosette Ricker
Feb 28, 2003·Biology of Reproduction·Sankha BhowmickJohn Biggers
Mar 26, 2003·Biotechnology and Bioengineering·Jason P AckerMehmet Toner
Apr 11, 2003·Cryobiology·Jason P Acker, Locksley E McGann

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Citations

Mar 16, 2012·Knee Surgery, Sports Traumatology, Arthroscopy : Official Journal of the ESSKA·Lovro SuhodolčanJanez Brecelj
Sep 18, 2010·Annals of Biomedical Engineering·Geer YangXiaoming He
Jan 28, 2010·Analytical Chemistry·Anurag BajpayeeMehmet Toner
Feb 16, 2010·Reproduction : the Official Journal of the Society for the Study of Fertility·Shogo HigakiYoshiyuki Takahashi
Aug 28, 2014·Biopreservation and Biobanking·Hai-Yan WangShu-Shen Lu
Nov 19, 2013·Journal of Endodontics·Su-Jin AhnSang Hyuk Park
Feb 22, 2012·Transfusion and Apheresis Science : Official Journal of the World Apheresis Association : Official Journal of the European Society for Haemapheresis·A StolzingS Sethe
Dec 3, 2011·American Journal of Orthodontics and Dentofacial Orthopedics : Official Publication of the American Association of Orthodontists, Its Constituent Societies, and the American Board of Orthodontics·Hiroko KamadaKazuo Tanne
Apr 29, 2011·Cryobiology·Tiziana IrdaniPio Federico Roversi
Jan 29, 2011·Fertility and Sterility·Enrique CriadoPaolo Emanuele Levi-Setti
Dec 7, 2010·Fertility and Sterility·Enrique CriadoPaolo Emanuele Levi-Setti
Mar 24, 2009·Cryobiology·Kenneth L RoachMehmet Toner
Apr 7, 2016·International Journal of Pharmaceutics·Géraldine BroeckxFilip Kiekens
Aug 6, 2015·Journal of Food Science and Technology·Siavash IravaniSeyed Vahid Mirmohammadi
Mar 19, 2013·Acta Biomaterialia·Hajira F Ahmad, Athanassios Sambanis
Sep 3, 2013·Cryobiology·Wei-Hung ShihWei-Te Wu
Aug 17, 2014·Cryobiology·Blake StokichMichael A Menze
May 21, 2014·Preparative Biochemistry & Biotechnology·He ChenGuowei Shu
Jul 6, 2014·Biotechnology Journal·Waseem AsgharUtkan Demirci
Dec 25, 2016·Carbohydrate Research·Madeleine K AdamBrendan L Wilkinson
Jul 16, 2015·Chemical Communications : Chem Comm·Daniel E MitchellMatthew I Gibson
Jan 31, 2012·Cells, Tissues, Organs·Sheng-Yang LeeWei-Chung Vivian Yang
Aug 25, 2017·Zygote : the Biology of Gametes and Early Embryos·Shogo HigakiTatsuyuki Takada
Nov 17, 2017·Nature Communications·Caroline I BiggsMatthew I Gibson

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