Cryoelectron microscopy and image analysis of the cardiac ryanodine receptor.

The Journal of Biological Chemistry
M R SharmaTerence Wagenknecht

Abstract

The three-dimensional structure of the cardiac muscle ryanodine receptor (RyR2) is described and compared with its skeletal muscle isoform (RyR1). Previously, structural studies of RyR2 have not been as informative as those for RyR1 because optimal conditions for electron microscopy, which require low levels of phospholipid, are destabilizing for RyR2. A simple procedure was devised for diluting RyR2 (in phospholipid-containing buffer) into a lipid-free buffer directly on the electron microscope grid, followed by freezing within a few seconds. Cryoelectron microscopy of RyR2 so prepared yielded images of sufficient quality for analysis by single particle image processing. Averaged projection images for RyR2, as well as for RyR1, prepared under the same conditions, were found to be nearly identical in overall dimensions and appearance at the resolution attained, approximately 30 A. An initial three-dimensional reconstruction of RyR2 was determined (resolution approximately 41 A) and compared with previously reported reconstructions of RyR1. Although they looked similar, which is consistent with the similarity found for the projection images, and with expectations based on the 66% amino acid sequence identity of the two isoforms,...Continue Reading

References

Jan 1, 1989·Annual Review of Biophysics and Biophysical Chemistry·S Fleischer, M Inui
Aug 1, 1988·Journal of Electron Microscopy Technique·M Radermacher
Dec 1, 1995·Journal of the Optical Society of America. A, Optics, Image Science, and Vision·W Liu, J Frank
Aug 1, 1995·FEBS Letters·T Wagenknecht, M Radermacher
Jan 28, 1994·Biochemical and Biophysical Research Communications·A P TimermanS Fleischer
Mar 1, 1993·Trends in Pharmacological Sciences·V Sorrentino, P Volpe
Jun 1, 1996·Nature Structural Biology·E V OrlovaW Chiu
Mar 28, 1997·The Journal of Biological Chemistry·T YamazawaM Iino
Apr 1, 1997·Current Opinion in Structural Biology·T Wagenknecht, M Radermacher
Jul 1, 1997·Biophysical Journal·J A CopelloS Fleischer
Jul 1, 1997·Physiological Reviews·C Franzini-Armstrong, F Protasi
Nov 1, 1970·The Journal of Cell Biology·C Franzini-Armstrong

❮ Previous
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Citations

Feb 7, 2008·European Biophysics Journal : EBJ·Irina BaranVirgil Baran
Jan 25, 2003·Cell Calcium·Daniela Rossi, Vincenzo Sorrentino
Jun 12, 2001·Current Opinion in Neurobiology·J Changeux, S J Edelstein
Jul 24, 2012·The Journal of Biological Chemistry·Filip Van Petegem
Oct 22, 2010·Cold Spring Harbor Perspectives in Biology·Johanna T LannerSusan L Hamilton
Sep 3, 2004·Cell Structure and Function·Eiji Notsu, Akira Matsuno
Nov 13, 2012·PloS One·Olga B PopovaIrina I Serysheva
May 17, 2001·Proceedings of the National Academy of Sciences of the United States of America·Z LiuT Wagenknecht
Aug 12, 2014·Archivos de cardiología de México·Angélica RuedaHéctor H Valdivia
Aug 26, 2014·Journal of Molecular and Cellular Cardiology·Christian Pinali, Ashraf Kitmitto
Jul 14, 2007·The Journal of Physiology·V LukyanenkoW J Lederer
Oct 12, 2010·Progress in Biophysics and Molecular Biology·Dong Woo SongDo Han Kim
Jul 26, 2011·Science China. Life Sciences·Lynn Kimlicka, Filip Van Petegem
Apr 10, 2009·Journal of Molecular Biology·Xing MengChang-Cheng Yin
Nov 25, 2014·Biophysical Journal·Bengt SvenssonRazvan L Cornea
Nov 25, 2014·Biophysical Journal·Suren A Tatulian
Aug 12, 2005·Annals of the New York Academy of Sciences·Long-Sheng SongW J Lederer
Mar 24, 2011·Journal of Cellular and Molecular Medicine·Mihaela Gherghiceanu, Laurentiu M Popescu
Jan 3, 2006·Biophysical Journal·Xiaoying KohAndre Levchenko
Jan 5, 2010·Protein Expression and Purification·Vladena Bauerová-HlinkováJozef Sevcík
Sep 18, 2002·Journal of Molecular and Cellular Cardiology·Silvia GuatimosimW J Lederer
Apr 7, 2009·Pharmacology & Therapeutics·Lynda M Blayney, F Anthony Lai
May 19, 2011·Journal of Molecular and Cellular Cardiology·M B Cannell, Cherrie H T Kong
Oct 11, 2005·Biophysical Journal·Manjuli Rani SharmaTerence Wagenknecht
Jun 23, 2016·Biophysical Journal·Vanessa CabraMontserrat Samsó
Aug 24, 2005·Cell Calcium·Susan L Hamilton
Nov 22, 2011·Journal of Molecular Biology·Sjors H W Scheres
Jan 10, 2008·Biochemical and Biophysical Research Communications·Sidney Fleischer
Apr 28, 2004·FEBS Letters·Ming-Chuan WangAshraf Kitmitto
Dec 31, 2003·The Journal of Biological Chemistry·Angela F DulhuntyMarco G Casarotto
Apr 18, 2000·FEBS Letters·M ManuntaH Schindler
Jan 15, 2004·The Journal of Biological Chemistry·Lynda M BlayneyF Anthony Lai
Nov 6, 2001·The Journal of Biological Chemistry·Montserrat Samsó, Terence Wagenknecht
Sep 25, 2002·Physiological Reviews·Michael Fill, Julio A Copello

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