PMID: 6987774Jan 1, 1980Paper

Cryofixation without cryoprotectants. Freeze substitution and freeze etching of an insect olfactory receptor

Tissue & Cell
R A Steinbrecht

Abstract

Antennae of the silk moth, Bombyx mori, were frozen by immersion into propane at -180 degrees C, and further processed by (a) freeze substitution (FS) or (b) freeze etching (FE). Although no cryoprotectant was used, freezing damage was observed in deeper tissue regions only. Data from FS specimens closely resemble those from FE replicas. Therefore, FS usually does not induce noticeable secondary artefacts by the preparation steps subsequent to freezing. When compared with chemically fixed antennae, the superior quality of cryofixation in this tissue is evident, particularly where cell surfaces and processes border the receptor lymph cavity; membranes are smooth following a steady course; dendrites and axons are round in cross-section with evenly distributed microtubules. The value of cryofixation is discussed with special reference to structures of presumed functional significance (e.g. stimulus conducting pore tubules, intramembrane particles of the receptor membrane, the ciliary segment of the dendrites, intercellular dilations, membrane junctions).

References

Mar 29, 1977·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·J Farrant
Jan 1, 1976·Tissue & Cell·R A Steinbrecht, B Müller
Sep 1, 1977·Journal of Microscopy·F Franks
Jan 1, 1978·Journal of Microscopy·P Echlin
Jan 1, 1976·Cold Spring Harbor Symposia on Quantitative Biology·J E HeuserD M Landis
Jul 1, 1975·The Anatomical Record·A V Harreveld, E Fifkova
Feb 1, 1975·Cryobiology·G E Van VenrooijH A Van der Vorst
Oct 3, 1975·Science·D BrantonR S Weinstein
Mar 1, 1976·Journal of Ultrastructure Research·D Kerjaschki, H Hörander
Jan 1, 1976·Journal of Insect Physiology·C E Phillips, J S Vande Berg
May 1, 1970·The Journal of Cell Biology·D A Goodenough, J P Revel
Aug 1, 1973·The Journal of Cell Biology·P Claude, D A Goodenough
Dec 1, 1973·European Journal of Clinical Pharmacology·M S BenedettiC Nagant de Deuxchaisnes
May 27, 1971·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·H Moor
May 27, 1971·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·D Branton
Jan 1, 1974·Journal of Neurobiology·R E Coggeshall
Mar 1, 1974·Journal of Microscopy·A Van HarreveldJ Steiner
Jan 1, 1974·International Review of Cytology·L A Staehelin
Jan 1, 1973·Annual Review of Entomology·P Satir, N B Gilula
Apr 1, 1972·The Journal of Cell Biology·H PlattnerL Bachmann
Feb 1, 1973·Canadian Journal of Microbiology·J E Gilchrist, I W DeVoe
Jul 1, 1974·Scientific American·D Schneider
Feb 1, 1970·Journal of Ultrastructure Research·E A Robertson, R L Schultz
Sep 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·L Bachmann, W W Schmitt
Jan 1, 1971·Zeitschrift für Zellforschung und mikroskopische Anatomie·R A Steinbrecht, B Müller
Mar 1, 1969·The Journal of Cell Biology·M W Brightman, T S Reese
Jun 1, 1967·The Journal of Cell Biology·J P Revel, M J Karnovsky
Aug 1, 1962·Journal of Ultrastructure Research·R F BAKER
Apr 1, 1964·Journal of Ultrastructure Research·F S SJOESTRAND, L G ELFVIN
Sep 1, 1964·The Journal of Cell Biology·W R LOEWENSTEIN, Y KANNO
Sep 1, 1964·The Journal of Cell Biology·J WIENERW R LOEWENSTEIN
Jul 1, 1964·The Anatomical Record·A VANHARREVELD, J CROWELL
Apr 1, 1965·The Journal of Cell Biology·A VANHARREVELDS K MALHOTRA

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Citations

Jun 28, 2005·Insect Biochemistry and Molecular Biology·Jonathan Bohbot, Richard G Vogt
Apr 1, 1997·Journal of Anatomy·T AkisakaM Inoue
Dec 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·R G VogtG D Prestwich
Apr 15, 1993·Microscopy Research and Technique·R A Steinbrecht
Sep 1, 1983·Journal of Neurobiology·J A WilsonB Müller
Jul 21, 2012·Arthropod Structure & Development·Thomas A Keil
Oct 31, 2015·Journal of Chemical Ecology·Yunpo ZhaoXuexia Miao
May 19, 2006·Biology of the Cell·Cveta TomovaBruno M Humbel
Apr 25, 2007·Traffic·Elly van DonselaarJan W Slot
Oct 1, 1984·Journal of Microscopy·R A Steinbrecht, K Zierold
Sep 10, 1981·Nature·R G Vogt, L M Riddiford
Mar 1, 1984·Journal of Morphology·Fran M van der WolkH Van Der Starre

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