Cryogenic sequenced layering for the 3D reconstruction of biological objects.

Scientific Reports
Vladimir Nikolaevich NikolenkoMikhail Yegorovich Sinelnikov

Abstract

Three-dimensional (3D) visualization is applied throughout many specialities, prompting an important breakthrough in accessibility and modeling of data. Experimental rendering and computerized reconstruction of objects has influenced many scientific achievements, facilitating one of the greatest advancements in medical education since the first illustrated anatomy book changed specialist training forever. Modern medicine relies on detailed, high quality virtual models for educational, experimental and clinical purposes. Almost all current virtual visualization methods rely on object slicing producing serial sections, which can then be digitalized or analyzed manually. The tendency to computerize serial sections roots from convenience, accessibility, decent visualization quality and automation capabilities. Drawbacks of serial section imaging is tissue damage occurring within each consequent sectioning. To utilize the important aspects of real-life object reconstruction, and maintain integrity of biological structures, we suggest a novel method of low-temperature layering of objects for digitization and computerized virtual reconstruction. Here we show the process of consequent imaging of each novel layer of a biological object,...Continue Reading

References

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Methods Mentioned

BETA
fluorescence microscopy
chemical treatment

Software Mentioned

Selena
VBIO
NET framework
VOXEL
TRIO
Modex
Statistica
Visible Human
Imaris
Microsoft Visual Studio

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