Cryogenic spinal cord injury induces astrocytic gene expression of insulin-like growth factor I and insulin-like growth factor binding protein 2 during myelin regeneration

Journal of Neuroscience Research
D L YaoH D Webster

Abstract

To study injury-induced astrocytic responses associated with regrowth of axons and regeneration of myelin, the method of Collins and colleagues was used to make focal cryogenic lesions in spinal cords of adult rats (Collins et al.: J Neuropathol Exp Neurol 45: 742-757, 1986). The duration of cryogenic injury (CI), the size of the cryode, and its temperature were chosen to destroy all myelin sheaths and axons without producing cavities or hemorrhages. Messenger RNA and peptide distributions of insulin-like growth factor I (IGF-I), IGF-I receptor (IGFR-I), IGF binding protein 2 (IGFBP-2), glial fibrillary acidic protein (GFAP), and myelin basic protein (MBP) were studied 3-56 days after CI by in situ hybridization and immunocytochemistry. At 3 days, vimentin-positive, GFAP-negative astrocyte-like cells in the lesion expressed IGF-I mRNA and peptide and 7 days after CI, both were expressed by typical GFAP-positive, hypertrophic astrocytes, many of which also were vimentin-positive. Levels of IGF-I, IGFBP-2, and GFAP mRNA and peptide were higher in lesion astrocytes after 14 days. They attained maximum levels at 21-28 days before declining to near control levels at 56 days. Decreasing relative levels of oligodendroglial MBP mRNA we...Continue Reading

References

Jan 31, 1992·Biochemical and Biophysical Research Communications·P GluckmanK Nikolics
Mar 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·S KomolyC A Bondy
Nov 15, 1991·The Journal of Comparative Neurology·J Gehrmann, G W Kreutzberg
Jan 1, 1991·Glia·M E HattenC A Mason
May 1, 1990·Brain Research. Molecular Brain Research·M BrennerE Freese
Sep 6, 1990·Nature·P R Lennard
Oct 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·H WernerD LeRoith
Jan 1, 1989·Brain Research Bulletin·G H Collins, N R West
Jan 1, 1989·Brain Research Bulletin·G H Collins, N R West
Jan 1, 1989·Journal of Neuropathology and Experimental Neurology·N R West, G H Collins
Aug 1, 1988·Journal of Neurocytology·M B GraeberG W Kreutzberg
Feb 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·F A McMorrisR W Furlanetto
Jan 1, 1987·Journal of Neuroscience Research·J R Connor, R E Fine
Nov 1, 1986·Journal of Neuropathology and Experimental Neurology·G H CollinsD A Ward
Nov 1, 1986·Journal of Neuropathology and Experimental Neurology·G H CollinsJ D Parmely
Oct 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·B BlochA Kahn
Apr 1, 1981·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·S M HsuH Fanger
May 1, 1994·Journal of Neuropathology and Experimental Neurology·M D Norenberg
Jan 1, 1994·Annual Review of Neuroscience·D M Landis
Feb 1, 1992·Molecular and Cellular Neurosciences·W H LeeC A Bondy

❮ Previous
Next ❯

Citations

Dec 1, 1996·Molecular Neurobiology·A J D'ErcoleG Gutierrez-Ospina
Sep 25, 2009·Cellular and Molecular Neurobiology·Panagiota MiltiadousFotini Stylianopoulou
Mar 16, 2011·Neurotherapeutics : the Journal of the American Society for Experimental NeuroTherapeutics·Akshata AlmadDana M McTigue
Feb 20, 1997·Brain Research. Developmental Brain Research·W ZhangW H Lee
Jul 1, 1998·Brain Research. Molecular Brain Research·J L CookN G Bazan
Sep 9, 2000·Forensic Science International : Synergy·A S DebertinW Götz
Aug 19, 1999·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·N B ChauhanT Khan
Jan 1, 1997·European Journal of Paediatric Neurology : EJPN : Official Journal of the European Paediatric Neurology Society·W Zumkeller
Jun 20, 1995·Proceedings of the National Academy of Sciences of the United States of America·D L YaoH D Webster
Jan 15, 1996·The Journal of Clinical Investigation·B M JohnstonP D Gluckman
Nov 15, 2012·Journal of Translational Medicine·Juan E Puche, Inma Castilla-Cortázar
Sep 22, 1999·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·M z ChengL E Lanyon
Apr 2, 2002·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Ming Zhao ChengLance E Lanyon
Jun 15, 2004·Neurobiology of Disease·Andrea M VincentEva L Feldman
Oct 20, 1998·Seminars in Pediatric Neurology·L L BillinghurstE Y Snyder
May 3, 2005·Brain Research. Developmental Brain Research·Marieke E van MeeterenEric A F van Tol
Nov 21, 2000·Molecular and Cellular Neurosciences·G L Hinks, R J Franklin
Feb 15, 2002·Brain : a Journal of Neurology·Guus Wolswijk
Sep 30, 2015·BioMed Research International·Annunziato MangiolaGiorgio Lofrese
Apr 14, 2017·Neurogenesis·Gustavo R MorelRodolfo G Goya
Apr 1, 1997·Multiple Sclerosis : Clinical and Laboratory Research·H D Webster
Oct 23, 2001·Neuropathology : Official Journal of the Japanese Society of Neuropathology·N R WestG H Collins

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cardiomyopathy

Cardiomyopathy is a disease of the heart muscle, that can lead to muscular or electrical dysfunction of the heart. It is often an irreversible disease that is associated with a poor prognosis. There are different causes and classifications of cardiomyopathies. Here are the latest discoveries pertaining to this disease.

Astrocytes in Repair & Regeneration

Astrocytes are glial cells found within the CNS and are able to regenerate new neurons. They become activated during CNS injury and disease. The activation leads to the transcription of new genes and the repair and regeneration of neurons. Discover the latest research on astrocytes in repair and regeneration here.

Astrocytes

Astrocytes are glial cells that support the blood-brain barrier, facilitate neurotransmission, provide nutrients to neurons, and help repair damaged nervous tissues. Here is the latest research.