PMID: 9662272Jul 14, 1998Paper

Cryohemolysis for the detection of hereditary spherocytosis: correlation studies with osmotic fragility and autohemolysis

American Journal of Hematology
S Streichman, Y Gescheidt

Abstract

Laboratory methods aimed to assess the presence of spheroidal cells such as osmotic fragility, autohemolysis, and glycerol lysis time are very elaborate, time consuming, and often give inconclusive results. We have developed a diagnostic test based on a unique sensitivity of HS cells to hypertonic cryohemolysis and analyzed blood samples of 55 HS patients. The patients were divided into two subgroups, clinically affected probands and their relatives. To get quantitative comparisons with the classic methods, the cryohemolysis results were compared to two parameters of the osmotic fragility test: the salt concentration that causes 50% hemolysis, and the percent lysis at a constant salt concentration. Autohemolysis results were also compared. To evaluate which of these tests has the best analytical power, we calculated the mean results and 2 SDs of each parameter in a control group, and then looked to see which of them was best in identifying the patients. The cryohemolysis test was the single parameter that identified all cases including asymptomatic carriers of the disease. The ability of this test to identify the less severe cases probably reflects the dependency of the cryohemolysis on factors that are more related to the prim...Continue Reading

References

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Citations

Jan 15, 2011·Clinical Chemistry and Laboratory Medicine : CCLM·France DebaugniesBéatrice Gulbis
Apr 6, 2012·Expert Review of Hematology·Constance GuillaudMarc Michel
Feb 8, 2011·La Presse médicale·Valentine LoustauMarc Michel
Aug 30, 2008·Cytometry. Part B, Clinical Cytometry·Dong Il Won, Jang Soo Suh
Mar 14, 2013·International Journal of Laboratory Hematology·M-J King, A Zanella
Sep 11, 2008·International Journal of Laboratory Hematology·R KarH P Pati
Jul 20, 2017·International Journal of Laboratory Hematology·A H Abdulsalam, Y Ghiath
Jun 11, 2014·Annals of Hematology·Elena LazarovaBéatrice Gulbis
Jun 18, 2019·Case Reports in Hematology·Kanda FanhchaksaiPimlak Charoenkwan
Aug 4, 2004·British Journal of Haematology·P H B Bolton-MaggsUNKNOWN General Haematology Task Force of the British Committee for Standards in Haematology
May 28, 2019·Orphanet Journal of Rare Diseases·Hyoung Soo ChoiUNKNOWN Hereditary Hemolytic Anemia Working Party of the Korean Society of Hematology
Dec 25, 2019·Biochimica Et Biophysica Acta. Biomembranes·Susana RochaAlice Santos-Silva

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