Abstract
Cryopreservation of mesenchymal stem cells from amniotic fluid is of clinical importance, as these cells can be harvested during the prenatal period and stored for use in treatments. We examined the behavior of mesenchymal stem cells from human amniotic fluid in culture that had been subjected to cryopreservation. We assessed chromosomal stability through karyotype analysis, determined whether multipotent capacity (differentiation into adipogenic, chondrogenic, and osteogenic cells) is maintained, and analyzed SOX2 and NANOG expression after thawing. Five amniotic fluid samples were cryopreserved for 150 days. No chromosomal aberrations were observed. The expression levels of NANOG and SOX2 also were quite similar before and after cryopreservation. Capacity for differentiation into adipogenic, chondrogenic, and osteogenic tissues also remained the same. We conclude that cryopreservation of amniotic fluid does not alter karyotype, NANOG/SOX2 gene expression, or multipotent capacity of stem cells that have been collected from amniotic fluid during pregnancy.
References
Oct 1, 1983·British Medical Bulletin·C M Gosden
Jan 1, 1982·Methods in Cell Biology·H Hoehn, D Salk
Feb 1, 1996·Biomaterials·J O Karlsson, M Toner
Jun 1, 1997·Clinical Obstetrics and Gynecology·R A Brace
Jun 1, 1997·Cryobiology·K R Diller
Apr 1, 1997·Revista da Associação Médica Brasileira·C M MassumotoF Dulley
Aug 2, 2000·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·J J MinguellA Erices
Mar 30, 2001·Bone Marrow Transplantation·O N Koç, H M Lazarus
Jul 2, 2003·Human Reproduction·Andrea-Romana PrusaMarkus Hengstschläger
Jul 15, 2003·Journal of Hematotherapy & Stem Cell Research·Erik J WoodsJohn K Critser
Apr 24, 2004·Human Reproduction·Ming-Song TsaiShiaw-Min Hwang
Apr 23, 2005·American Journal of Obstetrics and Gynecology·Andreina SchoeberleinDaniel V Surbek
Jul 1, 2005·Molecular and Cellular Biology·Joon-Lin ChewHuck-Hui Ng
Aug 6, 2005·Stem Cells·Toshio MikiStephen C Strom
Sep 13, 2005·Cell·Laurie A BoyerRichard A Young
Nov 25, 2005·Biology of Reproduction·Ming-Song TsaiYu-Jen Chang
Mar 7, 2006·Nature Genetics·Yuin-Han LohHuck-Hui Ng
Mar 15, 2006·BMC Cell Biology·Mandana Mohyeddin BonabBehrouz Nikbin
Apr 18, 2006·Cell Research·Patrizia BossolascoLorenza Lazzari
Jun 13, 2006·Nature·Natalia IvanovaIhor R Lemischka
Sep 19, 2006·PLoS Computational Biology·Vijay ChickarmaneCarsten Peterson
Jan 9, 2007·Nature Biotechnology·Paolo De CoppiAnthony Atala
Jan 11, 2007·Nature Biotechnology·Alan Trounson
Jan 18, 2007·Cell Proliferation·J KimJ You
Mar 3, 2007·Stem Cells·Dolores BakshRocky S Tuan
Sep 1, 2007·Stem Cells·Steven J GrecoPranela Rameshwar
Jan 16, 2008·Journal of Cellular and Molecular Medicine·Akiva J Marcus, Dale Woodbury
Jun 5, 2008·BMC Genomics·Alexei A SharovMinoru S H Ko
Citations
Jun 19, 2013·Cytotherapy·Alessia ColosimoBarbara Barboni
Jan 13, 2016·Journal of Tissue Engineering and Regenerative Medicine·Kar Wey YongBelinda Pingguan-Murphy
Aug 19, 2015·Biopreservation and Biobanking·Kar Wey YongBelinda Pingguan-Murphy
Mar 7, 2018·Regenerative Medicine·Kar Wey YongWan Kamarul Zaman Wan Safwani
Nov 17, 2017·Animal Biotechnology·Joanna RomanekZdzisław Smorąg
Aug 11, 2019·New Biotechnology·Jaromír VašíčekPeter Chrenek