Cryopreservation-induced alterations in protein composition of rainbow trout semen

Proteomics
Joanna NyncaAndrzej Ciereszko

Abstract

The aim of this study was to detect cryopreservation-induced alterations in the protein composition of rainbow trout semen using two independent methods 1DE SDS-PAGE prefractionation combined with LC-MS/MS and 2D difference gel electrophoresis followed by MALDI-TOF/TOF identification. Here, we show the first comprehensive dataset of changes in rainbow trout semen proteome after cryopreservation, with a total of 73 identified proteins released from sperm to extracellular fluid, including mitochondrial, cytoskeletal, nuclear, and cytosolic proteins. Our study provides new information about proteins released from sperm, their relation to sperm structure and function, and changes of metabolism of sperm cells as a result of cryopreservation. The identified proteins represent potential markers of cryoinjures of sperm structures and markers of the disturbances of particular sperm metabolic pathways. Further studies will allow to decipher the precise function of the proteins altered during rainbow trout cryopreservation and are useful for the development of extensive diagnostic tests of sperm cryoinjures and for the successful improvement of sperm cryopreservation of this economically important species.

References

Oct 13, 2001·Molecular Reproduction and Development·C LabbeG Maisse
Feb 26, 2003·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Katarzyna GrzybEdward F Skorkowski
Jan 22, 2005·Biology of Reproduction·Loredana ZilliSebastiano Vilella
Nov 1, 2013·Proteomics·Joanna NyncaAndrzej Ciereszko
Apr 2, 2014·Proteomics·Joanna NyncaAndrzej Ciereszko

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Citations

Jul 1, 2015·Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics·Mariola A DietrichAndrzej Ciereszko
Mar 29, 2019·Reproduction : the Official Journal of the Society for the Study of Fertility·J NyncaA Ciereszko
Apr 5, 2018·Scientific Reports·Anna Kur-PiotrowskaBarbara Gawronska-Kozak
Sep 20, 2019·International Journal of Molecular Sciences·Patricia Peris-FrauAna Josefa Soler
Apr 29, 2019·Theriogenology·Mariola A DietrichAndrzej Ciereszko
Aug 8, 2021·International Journal of Molecular Sciences·Agnieszka MostekAndrzej Ciereszko

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