Cryopreservation of articular cartilage. Part 2: mechanisms of cryoinjury

Cryobiology
David PeggC J Hunt

Abstract

Although isolated chondrocytes can be cryopreserved by standard methods, at the present time there is no satisfactory method that will preserve living chondrocytes in situ in surgical grafts, between the time of procurement or manufacture and actual use; survival of living chondrocytes in situ is inadequate at best and is also very variable. The first step in identifying the cause of this discrepancy was to establish that the cryoprotectants we had chosen to use, dimethyl sulphoxide and propylene glycol, do actually penetrate into the tissue rapidly. They do. Moreover, chondrocytes were shown to tolerate 10 or 20% Me2SO and were not unusually susceptible to osmotic stress. An experiment in which the effects of freezing with 10% Me2SO to -50 degrees C were separated from the effects of the concomitant rise in solute concentration showed that injury was associated with the formation of ice as such. Freeze substitution microscopy showed that large ice crystals were formed within the chondron, some at least within chondrocytes, even when the cooling rate was optimal for isolated chondrocytes. It is proposed that the nucleation and preferential growth of ice within the chondron (rather than the surrounding acellular matrix) is respo...Continue Reading

References

Aug 23, 1988·Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character·B Rubinsky, D E Pegg
Jan 1, 1988·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·C A PooleB W Beaumont
Aug 1, 1974·Cryobiology·A R HayesR E Kingston
Jul 1, 1970·Physics in Medicine and Biology·D E Pegg, A R Hayes
Jan 1, 1993·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·K TavakolN S Schachar
Aug 1, 1994·Cryobiology·E A BatesonM J Taylor
Feb 1, 1994·Cryobiology·K MuldrewL E McGann
May 1, 1996·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·C OhlendorfH J Mankin
Jul 1, 1997·Journal of Anatomy·C A Poole
Mar 13, 1956·Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character·R C CURRAN, T GIBSON

❮ Previous
Next ❯

Citations

Jul 21, 2011·Knee Surgery, Sports Traumatology, Arthroscopy : Official Journal of the ESSKA·R VillalbaJ L Gómez Villagrán
Sep 8, 2006·Cell and Tissue Banking·David E Pegg
Feb 1, 2013·Biopreservation and Biobanking·Avelina Sotres-VegaL Felipe Jiménez-García
Feb 1, 2013·Biopreservation and Biobanking·Xiaoyi YuShaozhi Zhang
Sep 4, 2013·Cell and Tissue Banking·Patrycja MickiewiczZygmunt Wróbel
Aug 28, 2014·Biopreservation and Biobanking·Hai-Yan WangShu-Shen Lu
Jun 5, 2012·Cryobiology·Issei OnariHiroyuki Tsuchiya
Dec 21, 2007·Cryobiology·Monica C WustemanDavid E Pegg
Feb 16, 2007·Cryobiology·Zhang Shaozhi, David E Pegg
Dec 21, 2006·Biomaterials·L L KuleshovaD W Hutmacher
Dec 7, 2014·Cell and Tissue Banking·Eduardo Branco de SousaBeni Olej
Oct 25, 2011·Cryobiology·Gaye Cetinkaya, Sezen Arat
Mar 19, 2013·Cryobiology·Alireza AbazariLocksley E McGann
Aug 16, 2016·Cell and Tissue Banking·Wichaya SriutthaPeraphan Pothacharoen
Mar 8, 2017·Current Opinion in Organ Transplantation·Bote G Bruinsma, Korkut Uygun
Feb 6, 2014·The American Journal of Sports Medicine·Harold E HuntWilliam D Bugbee
Mar 1, 2012·Journal of Zhejiang University. Science. B·Shao-Zhi ZhangGuang-Ming Chen
Nov 30, 2018·Biopreservation and Biobanking·Ariadna CorralRamón Risco
Jul 8, 2019·Current Opinion in Biotechnology·Reinier J de VriesKorkut Uygun
May 26, 2020·Cryobiology·Larissa LautnerJason P Acker

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.