Cryopreservation of articular cartilage. Part 3: the liquidus-tracking method

Cryobiology
David PeggDavid Vaughan

Abstract

Although it is relatively straightforward to cryopreserve living isolated chondrocytes, at the present time there is no satisfactory method to preserve surgical grafts between the time of procurement or manufacture and actual use. In earlier papers we have established that the cryoprotectants dimethyl sulphoxide or propylene glycol do penetrate into this tissue very rapidly. Chondrocytes are not unusually susceptible to osmotic stress; in fact they appear to be particularly resistant. It appears that damage is associated with the formation of ice per se, even at cooling rates that are optimal for the cryopreservation of isolated chondrocytes. We then showed that current methods of cartilage cryopreservation involve the nucleation and growth of ice crystals within the chondrons rather than ice being restricted to the surrounding acellular matrix. This finding established the need to avoid the crystallization of ice--in other words, vitrification. Song and his colleagues have published a vitrification method that is based on the use of one of Fahy's vitrification formulations. We confirmed the effectiveness of this method but found it to be very dependent on ultra rapid warming. However, we were able to develop a 'liquidus-tracki...Continue Reading

References

Jul 1, 1970·Physics in Medicine and Biology·D E Pegg, A R Hayes
Jun 14, 2002·Cryobiology·Monica C WustemanPaul Fitch
Mar 13, 1956·Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character·R C CURRAN, T GIBSON
Apr 20, 2004·Cryobiology·Gregory M FahyEric Zendejas
Jun 19, 2004·Journal of Investigative Surgery : the Official Journal of the Academy of Surgical Research·Ying C SongKelvin G M Brockbank

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Citations

Jul 21, 2011·Knee Surgery, Sports Traumatology, Arthroscopy : Official Journal of the ESSKA·R VillalbaJ L Gómez Villagrán
Feb 1, 2013·Biopreservation and Biobanking·Avelina Sotres-VegaL Felipe Jiménez-García
Feb 1, 2013·Biopreservation and Biobanking·Xiaoyi YuShaozhi Zhang
Aug 28, 2014·Biopreservation and Biobanking·Hai-Yan WangShu-Shen Lu
Oct 14, 2010·Cryobiology·Nadr M JomhaLocksley E McGann
Dec 23, 2009·Cryobiology·Kelvin G M BrockbankYing C Song
Jun 23, 2009·Cryobiology·Brian Wowk
Dec 2, 2008·Cryobiology·Nadr M JomhaLocksley E McGann
Dec 21, 2007·Cryobiology·Monica C WustemanDavid E Pegg
Sep 29, 2007·Cryobiology·A BaudotJ Lornage
Dec 21, 2006·Biomaterials·L L KuleshovaD W Hutmacher
Mar 1, 2012·Journal of Zhejiang University. Science. B·Shao-Zhi ZhangGuang-Ming Chen
Oct 25, 2011·Cryobiology·Gaye Cetinkaya, Sezen Arat
Mar 19, 2013·Cryobiology·Alireza AbazariLocksley E McGann
Aug 16, 2016·Cell and Tissue Banking·Wichaya SriutthaPeraphan Pothacharoen
Aug 7, 2018·Current Opinion in Organ Transplantation·Laura C BurlageGerald Brandacher
Dec 29, 2019·Scientific Reports·Ellen Cristina Rivas LeonelChristiani A Amorim
Mar 21, 2021·Npj Regenerative Medicine·Kezhou WuNadr M Jomha
May 26, 2020·Cryobiology·Larissa LautnerJason P Acker
Jun 28, 2019·Transfusion Medicine and Hemotherapy : Offizielles Organ Der Deutschen Gesellschaft Fur̈ Transfusionsmedizin Und Immunham̈atologie·Michael J TaylorSebastian E Giwa

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