Cryopreservation of Atlantic cod Gadus morhua L. spermatozoa: Effects of extender composition and freezing rate on sperm motility, velocity and morphology

Cryobiology
Ian A E ButtsE A Trippel

Abstract

Broodstock selection programs are currently underway for Atlantic cod (Gadus morhua). To complement and further these selection programs we need to develop sperm cryopreservation procedures. This will allow genomic DNA from males from selected individuals or stocks to be frozen and conserved in perpetuity. In our study we used a full factorial ANOVA design to examine the effects of diluent (Mounib's sucrose-based diluent, Hanks' Balanced Salt Solution, Mounib's sucrose-based diluent+hen's egg yolk, and Hanks' Balanced Salt Solution+hen's egg yolk), cryoprotectant (propylene glycol, dimethyl sulphoxide, and glycerol), and freezing rate (-2.5, -5.0, -7.5, and -10.0°C/min) on motility of cod frozen-thawed sperm. Sperm velocity and morphometric analyses of sperm heads and flagella were also assessed. We found that sperm motility-recovery index was strongly influenced by the presence of higher-order interactions of the factors we tested. The best cryoprotection used diluents that contained hen's egg yolk. Generally, extenders containing propylene glycol yielded higher post-thaw sperm motilities than those with dimethyl sulphoxide or glycerol. In comparison to sperm from other frozen-thawed extenders, sperm from extenders supplemente...Continue Reading

References

May 1, 1978·Journal of Reproduction and Fertility·M S Mounib
Oct 27, 1998·Cryobiology·E CabritaM P Herraez
May 27, 2006·Theriogenology·C DreannoR Billard

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Citations

Feb 2, 2011·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Ian Anthony Ernest ButtsIgor Babiak
Oct 4, 2011·Theriogenology·I A E ButtsR M Rideout
Jun 19, 2016·General and Comparative Endocrinology·Juan F AsturianoÁkos Horváth
Apr 6, 2019·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Vahid ZadmajidIan Anthony Ernest Butts

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