Cryopreservation of DNA Origami Nanostructures.

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Yang XinAdrian Keller

Abstract

Although DNA origami nanostructures have found their way into numerous fields of fundamental and applied research, they often suffer from rather limited stability when subjected to environments that differ from the employed assembly conditions, that is, suspended in Mg2+ -containing buffer at moderate temperatures. Here, means for efficient cryopreservation of 2D and 3D DNA origami nanostructures and, in particular, the effect of repeated freezing and thawing cycles are investigated. It is found that, while the 2D DNA origami nanostructures maintain their structural integrity over at least 32 freeze-thaw cycles, ice crystal formation makes the DNA origami gradually more sensitive toward harsh sample treatment conditions. Whereas no freeze damage could be detected in 3D DNA origami nanostructures subjected to 32 freeze-thaw cycles, 1000 freeze-thaw cycles result in significant fragmentation. The cryoprotectants glycerol and trehalose are found to efficiently protect the DNA origami nanostructures against freeze damage at concentrations between 0.2 × 10-3 and 200 × 10-3 m and without any negative effects on DNA origami shape. This work thus provides a basis for the long-term storage of DNA origami nanostructures, which is an impo...Continue Reading

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Citations

Mar 1, 2020·Angewandte Chemie·Adrian Keller, Veikko Linko
Aug 31, 2020·Advanced Healthcare Materials·Xiaohui WuBaoquan Ding
Aug 25, 2020·Chemistry : a European Journal·Huimin WangXiaoqing Liu
Dec 9, 2020·Sensors·Shuang WangYe Tian
Feb 23, 2021·Advanced Nanobiomed Research·David M Smith, Adrian Keller
Mar 17, 2021·ACS Applied Materials & Interfaces·Jiahao FangLiang Zhong
Mar 30, 2021·Chemistry : a European Journal·Yang XinAdrian Keller
Aug 28, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yang XinAdrian Keller

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