PMID: 3769515Oct 1, 1986Paper

Cryopreservation of dog platelets with dimethyl sulfoxide: therapeutic effectiveness of cryopreserved platelets in the treatment of thrombocytopenic dogs, and the effect of platelet storage at -80 degrees C

Cryobiology
C R ValeriJ J Vecchione

Abstract

Dog platelets were frozen with 6% dimethyl sulfoxide at 2-3 degrees C per minute in a -80 degrees C mechanical freezer. The frozen platelets were stored at -80 degrees C for as long as 39 months. After storage at -80 degrees C for less than 1 year, platelet in vitro freeze-thaw-wash recovery values were 70%, and in vivo survival values 1 to 2 hr after transfusion were 40% those of fresh platelets. After 2 years or longer storage, in vitro freeze-thaw-wash recovery values were 60%, and in vivo survival values 1 to 2 hr after transfusion were 20% those of fresh platelets. These results indicate that significant deterioration of the dog platelets occurred between the first and second year of storage at -80 degrees C. Platelets that were stored frozen at -80 degrees C for less than 1 year and washed before transfusion into lethally irradiated thrombocytopenic dogs were hemostatically effective.

References

May 1, 1979·Transfusion·T J ContrerasC R Valeri
Jan 1, 1976·Vox Sanguinis·B K KimM G Baldini
Jan 1, 1973·Proceedings of the Society for Experimental Biology and Medicine·B K Kim, M G Baldini
May 1, 1964·The Journal of Clinical Investigation·R H ASTER, J H JANDL

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Citations

Dec 3, 2008·American Journal of Veterinary Research·Julien GuillauminFern Tablin
Oct 14, 2009·Journal of Veterinary Emergency and Critical Care·Mary Beth CallanBruce S Sachais
Oct 16, 2012·Journal of Veterinary Internal Medicine·K E JandreyM B Brooks
Sep 29, 2012·Journal of Veterinary Emergency and Critical Care·Bryan D Hux, Linda G Martin
Dec 21, 2010·Journal of Veterinary Emergency and Critical Care·Julien GuillauminFern Tablin
Jan 30, 2009·Journal of Veterinary Internal Medicine·E H ApplemanM B Callan
Jul 5, 2016·Journal of Veterinary Emergency and Critical Care·Zenithson Y NgJoe W Bartges

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