Cryopreservation of embryogenic tissues from mature holm oak trees

Cryobiology
Azahara Barra-JiménezMariano Toribio

Abstract

The development of a vitrification method for cryopreservation of embryogenic lines from mature holm oak (Quercus ilex L.) trees is reported. Globular embryogenic clusters of three embryogenic lines grown on gelled medium, and embryogenic clumps of one line collected from liquid cultures, were used as samples. The effect of both high-sucrose preculture and dehydration by incubation in the PVS2 solution for 30-90 min, on both survival and maintenance of the differentiation ability was evaluated in somatic embryo explants with and without immersion into liquid nitrogen. Growth recovery of the treated samples and ability to differentiate cotyledonary embryos largely depended on genotype. Overall, high growth recovery frequencies on gelled medium and increase of fresh weight in liquid medium were obtained in all the tested lines, also after freezing. However, the differentiation ability of the embryogenic lines was severely hampered following immersion into LN. Two of the embryogenic lines from gelled medium were able to recover the differentiation ability, one not. In the lines with reduced or no differentiation ability, variation in the microsatellite markers was observed when comparing samples taken prior to and after cryopreser...Continue Reading

References

Jul 1, 1995·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·B D DowH F Howe

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Citations

Jul 26, 2016·Frontiers in Plant Science·Yuan GuanZhen-Hong Su
Jul 11, 2016·Cryobiology·Namrata KumariRaghubansh Kumar Singh
Apr 11, 2019·Frontiers in Plant Science·María Teresa MartínezElena Corredoira

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