PMID: 6981655Jul 1, 1982Paper

Cryopreservation of human mononuclear cells for quality control in clinical immunology. I. Correlations in recovery of K- and NK-cell functions, surface markers, and morphology

Journal of Clinical Immunology
D M StrongR B Herberman

Abstract

Cryopreserved human peripheral blood mononuclear cells (PBMC) were tested for natural killer (NK) and antibody-dependent cellular cytotoxicity (ADCC) and for high-affinity (29 degrees C) and total (4 degrees C) rosette formation with sheep erythrocytes. PBMC produced variable NK activity following freezing and thawing, but consistently reacted well in ADCC. A significant correlation was found between low NK activity and a decreased percentage of low-affinity rosette-forming cells. On the contrary, the number of large granular lymphocytes (LGL), among which NK cells are restricted, and the reactivity with the monoclonal antibody OKT10, which recognizes the majority of LGL in the peripheral blood, were not significantly altered by cryopreservation. Cryopreserved cells proved to be excellent controls for determining the day-to-day variability of the NK assay and for selecting optimum conditions for this test in the clinical immunology laboratory.

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Citations

Mar 30, 1984·Journal of Immunological Methods·H F Pross, J A Maroun
Dec 20, 1989·Journal of Immunological Methods·I G Schmidt-WolfN J Chao
May 1, 1990·Immunopharmacology·H K WebsterC S Pavia
Jun 1, 1990·Veterinary Immunology and Immunopathology·R E TruaxM J Newman
Jan 1, 1989·The American Review of Respiratory Disease·D J TollerudR N Hoover
Jul 1, 1990·Clinical Immunology and Immunopathology·D J TollerudR N Hoover
Apr 1, 2008·Hematology/oncology and Stem Cell Therapy·Huda AlsayedFaisal Al Rawas
Sep 1, 1996·Nursing Research·J M ZellerM A Colletti
Mar 12, 2005·Human Reproduction·Raj RaiGeoffrey Trew

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