Cryopreservation of human spermatozoa: correlations of ultrastructural sperm head configuration with sperm motility and ability to penetrate zona-free hamster ova

Fertility and Sterility
P C SerafiniR P Marrs

Abstract

Freezing and storage of human male gametes is associated with a reduction in the overall semen quality and establishment of pregnancy. This study was done to evaluate the integrity of sperm head ultrastructure (SHU) with computerized and vapor freezing. Comparisons were made between the effect of cryopreservatives glycerol (G) and dimethylsulfoxide (DMSO) on SHU. Twelve ejaculates from five proven fertile donors were studied with the use of routine semen analysis, zona-free hamster ova, and SHU. Both cooling processes, regardless of the preservative used, significantly reduced sperm function and the number of SH with intact plasma membranes. The staged cooling technique was substantially superior to vapor freezing in all parameters analyzed (P less than 0.01). G was less detrimental to the postthaw SHU than 1 M DMSO. A significant positive correlation (r = 0.98; P less than 0.01) was noted between the total number of intact SH and motile sperm. Computerized freezing in a G-diluted semen rendered a sperm environment that allowed the highest number of forms with intact SH membranes and with the best chances to penetrate zona-free hamster ova.

Citations

Dec 29, 2000·Theriogenology·J V Abraham-PeskirE Uggerhøj
Jan 13, 2001·Molecular and Cellular Endocrinology·S OehningerM Morshedi
May 19, 2000·European Journal of Obstetrics, Gynecology, and Reproductive Biology·P StanicE Suchanek
Jan 5, 2002·Theriogenology·C M O MedeirosJ L Rodrigues
Dec 5, 2019·Cell and Tissue Banking·Maryam EzzatiMaryam Pashaiasl
Jan 1, 1988·Archives of Andrology·J Auger, J P Dadoune
Jul 28, 2020·Human Fertility : Journal of the British Fertility Society·Séphora C QueirozFernando M Reis
Jul 3, 2021·Journal of Clinical Medicine·Kaan Aydos, Oya Sena Aydos

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