Cryopreservation of metaphase II human oocytes effects mitochondrial membrane potential: implications for developmental competence

Human Reproduction
Amy JonesAndrew A Toledo

Abstract

Current outcome results with embryos derived from thawed MII human oocyes are significantly lower than with embryos cryopreserved at the pronuclear stage. Here, we investigated whether freezing-thawing was associated with changes in oocyte mitochondrial polarity (DeltaPsim) that could influence competence by altering ATP levels or the ability of the cytoplasm to regulate intracellular Ca2+. Fresh and thawed uninseminated and unfertilized MII oocytes were stained with the DeltaPsim-specific probe JC-1 to detect clusters of high-polarized mitochondria (J-aggregate positive) and with the Ca2+- specific probe Fluo-4 to measure changes in intracellular levels of this cation. ATP content per oocyte was measured directly and cortical granules were visualized with a cortical granule-specific probe. A significant difference between fresh and thawed MII oocytes existed for pericortical J-aggregate fluorescence and for the ability of the cytoplasm to increase free Ca2+ in response to ionophore exposure. No significant difference in ATP contents was measured and cryopreservation was not associated with an apparent release of cortical granules. Irreversible loss of high DeltaPsim in thawed oocytes may be associated with defects in Ca2+ sign...Continue Reading

Citations

Dec 6, 2011·Journal of Assisted Reproduction and Genetics·Amanda Souza SettiEdson Borges
Aug 24, 2012·Journal of Assisted Reproduction and Genetics·Cui ChenGuoning Huang
Dec 19, 2012·Journal of Assisted Reproduction and Genetics·Deirdre Zander-FoxMichelle Lane
Jun 20, 2013·Journal of Assisted Reproduction and Genetics·Natalie A Clark, Jason E Swain
Jun 24, 2006·Reproductive Biomedicine Online·S ChamayouA Guglielmino
Jun 14, 2005·Reproductive Biomedicine Online·S J Paynter
Jun 9, 2009·Zygote : the Biology of Gametes and Early Embryos·Alessia NicoliGiovanni B La Sala
Aug 15, 2009·Zygote : the Biology of Gametes and Early Embryos·J WangX J He
Aug 27, 2011·Zygote : the Biology of Gametes and Early Embryos·Y LiuQ Xing
May 26, 2009·Human Reproduction·J J BromfieldD F Albertini
Feb 10, 2011·Human Reproduction·Javier I GarcíaLuis Noriega-Hoces
Jul 26, 2011·Human Reproduction·M G LarmanD K Gardner
Oct 17, 2013·Human Reproduction·Giovanni CoticchioDavid F Albertini
Dec 25, 2007·BMC Developmental Biology·Jörg P BurgstallerRalf Steinborn
Oct 31, 2006·Reproduction : the Official Journal of the Society for the Study of Fertility·T TharasanitT A E Stout
Aug 31, 2004·Reproduction : the Official Journal of the Society for the Study of Fertility·Jonathan Van Blerkom
May 5, 2006·Reproduction : the Official Journal of the Society for the Study of Fertility·Jonathan Van BlerkomPatrick Davis
Sep 26, 2007·Molecular Human Reproduction·Jonathan Van Blerkom, Patrick Davis
Apr 17, 2014·BioMed Research International·In-Sul Hwang, Shinichi Hochi
Nov 13, 2010·Fertility and Sterility·Somjate ManipalviratnAlan DeCherney
Sep 4, 2008·Reproductive Biomedicine Online·S A NottolaA Borini
Jul 4, 2009·Reproductive Biomedicine Online·Alex C VargheseAshok Agarwal
Apr 17, 2008·Reproductive Biomedicine Online·Jonathan Van Blerkom
Apr 13, 2005·Reproductive Biomedicine Online·Laura RienziErmanno Greco
Mar 9, 2010·Critical Reviews in Oncology/hematology·Raymond Manohar Anchan, Elizabeth Sarah Ginsburg
Aug 12, 2008·Fertility and Sterility·Zsolt P NagyHilton I Kort
Mar 28, 2008·Fertility and Sterility·Roberto GualtieriRiccardo Talevi
Nov 6, 2007·Fertility and Sterility·Mandy G Katz-JaffeDavid K Gardner
Jun 25, 2009·Molecular Reproduction and Development·Xue-Ming ZhaoShi-En Zhu
Jan 31, 2006·The Australian & New Zealand Journal of Obstetrics & Gynaecology·Catharyn J SternJohn F Seymour
Oct 20, 2006·Theriogenology·David K GardnerMark G Larman
Jun 8, 2006·Fertility and Sterility·Yehudith GhetlerRuth Shalgi
Feb 5, 2014·The Journal of Reproduction and Development·Seul-Ki LeeXiang-Shun Cui

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