Feb 12, 2020

Cryopreservation of midbrain dopaminergic neural cells differentiated from human embryonic stem cells

bioRxiv
Nicola J DrummondTilo Kunath

Abstract

Recent advancements in protocols to differentiate human pluripotent stem cells into midbrain dopaminergic (mDA) neurons has improved the ability to model Parkinson's disease (PD) in a dish, and has provided a scalable source of donor cells for emerging PD cell replacement therapy (CRT). However, to facilitate reproducibility, collaboration, and clinical trials it would be highly beneficial to cryopreserve committed mDA neural precursors cells in a ready-to-use format. In terms of cell manufacturing for PD CRT trials, a cryopreserved transplantation-ready mDA cell product would provide a critical opportunity for quality control, efficacy testing, and safety assessments. To address this challenge, we have compared six (6) different clinical-grade cryopreservation media and different freezing conditions for mDA neural precursor cells differentiated from two human embryonic stem cell (ESC) lines, MasterShef7 and RC17. Significant differences in cell viability were observed at 24h post-thawing, but no differences were observed immediately upon thawing. This highlights the need to check cell viability over the first 24h after thawing, and that viability of freshly thawed cells is insufficient to gauge the success of a cryopreservatio...Continue Reading

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Mentioned in this Paper

Pluripotent Stem Cells
Neural Stem Cells
Neurons
Dopaminergic Neurons
Cell Survival
ROCK1
Apoptosis
Cryopreservation
Cell Line, Tumor
Grade

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