Cryopreservation of Paramecium bursaria Chlorella Virus-1 during an active infection cycle of its host

PloS One
Samantha R CoySteven W Wilhelm

Abstract

Best practices in laboratory culture management often include cryopreservation of microbiota, but this can be challenging with some virus particles. By preserving viral isolates researchers can mitigate genetic drift and laboratory-induced selection, thereby maintaining genetically consistent strains between experiments. To this end, we developed a method to cryopreserve the model, green-alga infecting virus, Paramecium bursaria Chlorella virus 1 (PBCV-1). We explored cryotolerance of the infectivity of this virus particle, whereby freezing without cryoprotectants was found to maintain the highest infectivity (~2.5%). We then assessed the cryopreservation potential of PBCV-1 during an active infection cycle in its Chlorella variabilis NC64A host, and found that virus survivorship was highest (69.5 ± 16.5%) when the infected host is cryopreserved during mid-late stages of infection (i.e., coinciding with virion assembly). The most optimal condition for cryopreservation was observed at 240 minutes post-infection. Overall, utilizing the cell as a vehicle for viral cryopreservation resulted in 24.9-30.1 fold increases in PBCV-1 survival based on 95% confidence intervals of frozen virus particles and virus cryopreserved at 240 minut...Continue Reading

References

Sep 1, 1968·Journal of Virology·C Wallis, J L Melnick
Sep 1, 1984·The American Journal of Physiology·P Mazur
Mar 15, 2000·Journal of Virological Methods·C P BrussaardG Bratbak
Jun 24, 2003·Cryobiology·Zdenek Hubálek
Jun 28, 2003·Biophysical Journal·Amado CordovaAvinoam Ben-Shaul
Mar 10, 2004·Applied and Environmental Microbiology·Corina P D Brussaard
Mar 7, 2006·Virus Research·David D DuniganJames L Van Etten
Nov 18, 2006·Trends in Genetics : TIG·Jonathan FiléeMick Chandler
Apr 19, 2007·Cellular and Molecular Life Sciences : CMLS·W H RoosG J L Wuite
Apr 15, 1983·Virology·J L Van EttenR H Meints
Oct 16, 2009·Journal of Virology·Giane M Yanai-BalserJames L Van Etten
Jul 28, 2010·Journal of Virology·Rafael SanjuánRobert Belshaw
Feb 24, 2012·PloS One·Timo WulfmeyerTobias Meckel
Jul 3, 2013·Archives of Virology·Philippe ColsonDidier Raoult
Sep 24, 2013·Cryobiology·Raquel AmaralLília M A Santos
Mar 5, 2014·Proceedings of the National Academy of Sciences of the United States of America·Matthieu LegendreJean-Michel Claverie
Sep 23, 2014·Virology·Irina AgarkovaJames L Van Etten
Sep 10, 2015·Proceedings of the National Academy of Sciences of the United States of America·Matthieu LegendreJean-Michel Claverie
Nov 17, 2015·Journal of Virological Methods·Jun-Sun ParkSu Yeon Kim
Feb 1, 2008·Journal of Phycology·Janet M RoweSteven W Wilhelm
Mar 2, 2017·Archives of Virology·Csaba Kerepesi, Vince Grolmusz
May 13, 2017·The ISME Journal·William H WilsonSteven W Wilhelm
May 4, 2018·Journal of Virology·Kayla M Peck, Adam S Lauring

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Citations

Mar 26, 2021·Nature Reviews. Microbiology·Adrienne M S CorreaJoshua S Weitz

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Methods Mentioned

BETA
flow-cytometry

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