Cryopreservation of preantral ovarian follicles in situ from domestic cats (Felis catus) using different cryoprotective agents

Theriogenology
Ana Kelen F LimaLúcia D M Silva

Abstract

The objective of this investigation was to verify the structural characteristics of preantral follicles (PAF) of cat ovarian tissue after cryopreservation in 1.5 M glycerol or ethylene glycol, using a slow-freezing procedure. Ovaries (n = 10) from domestic cats were divided into fragments. One fragment was immediately preserved for classical histology (fresh control), and additional fragments were immersed in minimum essential medium plus 10% bovine fetal serum (MEM+BFS), or MEM+BFS supplemented with 1.5 M glycerol or ethylene glycol. The samples were frozen and plunged into liquid nitrogen. After 1 wk, the samples were thawed. A total of 600 PAF were evaluated. In the fresh control, there were 71.3% normal PAF. After thawing, the rates of normal PAF were 26.0, 39.3 and 58.0% for samples without cryoprotectant or with glycerol or ethylene glycol, respectively. We concluded that ethylene glycol was useful for the cryopreservation of feline PAF in situ.

References

Jul 1, 1994·The Veterinary Quarterly·S C HulshofR van den Hurk

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Citations

Feb 2, 2016·Biopreservation and Biobanking·Danielle C BritoJulio C Pieczarka
Jan 4, 2013·Reproduction in Domestic Animals = Zuchthygiene·G C Luvoni
Sep 29, 2011·Reproduction in Domestic Animals = Zuchthygiene·G C LuvoniS C Modina
Dec 17, 2014·Lasers in Medical Science·Matheus Franco da FrotaFabio Luis Camargo Villela Berbert
Nov 27, 2018·Reproduction in Domestic Animals = Zuchthygiene·Camila M GorrichoGilson H Toniollo
Dec 8, 2019·Animals : an Open Access Journal From MDPI·Janice M V VilelaCarolina M Lucci
Mar 11, 2016·Reproductive Medicine and Biology·Lalitha Devi, Sandeep Goel
Jul 8, 2018·Cryobiology·Ellen Cristina Rivas LeonelCarolina Madeira Lucci
May 15, 2021·Zygote : the Biology of Gametes and Early Embryos·D C C BritoA P R Rodrigues

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