Abstract
We describe experiments on the cryopreservation of the rabbit common carotid artery aimed at improving upon previous results. We describe the design of a double clamp which holds the artery during transportation and storage, preventing twisting, shortening, and collapse of the vessel. The device allowed perfusion with solutions as desired and markedly reduced the extent of endothelial loss during procurement and processing. We also studied the effects of three vehicle solutions; a modified Hanks' solution, a solution originally developed for the cryopreservation of smooth muscle (K-Pipes), and a solution designed for corneal endothelium (CP-Tes). The criteria used to make the assessments were smooth muscle contractility and the structure and function of the vascular endothelium. A new staining method for vascular endothelium (combining propidium iodide with silver nitrate) is described. We found that there was significantly more endothelial cell damage in rabbit carotid arteries frozen in Hanks' solution than in the other solutions, and the recovery of smooth muscle contractility was lowest in the Hanks' group. Arteries cryopreserved using CP-Tes as the vehicle solution showed less endothelial cell damage than arteries preserve...Continue Reading
Citations
Jan 9, 2007·Annals of Biomedical Engineering·Shannon L M DahlLaura E Niklason
Mar 22, 2006·Tissue Engineering·Shannon L M DahlYing C Song
May 28, 2010·Tissue Engineering. Part a·Stacey C SchutteRobert M Nerem
Dec 19, 2012·Journal of the Mechanical Behavior of Biomedical Materials·Bumsoo HanFrederick Grinnell
Dec 24, 2004·Cryobiology·Gemma PascualJulia Buján
Dec 26, 2001·Cryobiology·J BujánJ M Bellón
Jan 24, 2006·The Journal of Surgical Research·Peitao WangXing Li Wang
May 11, 2000·Journal of Vascular Surgery·M RigolG Sanz
Mar 4, 1999·European Journal of Vascular and Endovascular Surgery : the Official Journal of the European Society for Vascular Surgery·J M BellónJ Buján
Mar 24, 2000·The Journal of Surgical Research·F Arnaud
Feb 18, 2004·World Journal of Gastroenterology : WJG·Song GuJun-Hao Chen
Mar 4, 2000·Nature Biotechnology·Y C SongM J Taylor
Aug 8, 2018·Organogenesis·Gesine Pless-PetigUrsula Rauen
Oct 26, 2006·Cell and Tissue Banking·G PasquinelliR Conte
Nov 9, 2000·Journal of Investigative Surgery : the Official Journal of the Academy of Surgical Research·Y C SongK G Brockbank
Feb 26, 2021·Cell and Tissue Banking·Meredith StadnykNadr M Jomha
Nov 26, 2002·Cryobiology·Simona C Baicu, Michael J Taylor