Cryopreservation of UVC pathogen-inactivated platelets

Transfusion
Lauren WatersL Johnson

Abstract

Extending the platelet (PLT) shelf life and enhancing product safety may be achieved by combining cryopreservation and pathogen inactivation (PI). Although studied individually, limited investigations into combining these treatments has been performed. The aim of this study was to investigate the effect of PI treating PLTs before cryopreservation on in vitro PLT quality and function. ABO-matched buffy coat-derived PLTs in PLT additive solution (SSP+; Macopharma) were pooled and split to form matched pairs (n = 8). One unit remained untreated and the other was treated with the THERAFLEX UV-Platelets System (UVC; Macopharma). For cryopreservation, 5% to 6% dimethyl sulfoxide was added to the PLTs, and they were frozen at -80°C. After being thawed, untreated cryopreserved PLTs (CPPs) and UVC-treated CPPs (UVC-CPPs) were resuspended in plasma. In vitro quality was assessed immediately after thawing and after 24 hours of room temperature storage. UVC-CPPs had lower in vitro recovery compared to CPPs. By flow cytometry, PLTs demonstrated a similar abundance of GPIX (CD42a), GPIIb (CD41a), and GPIbα (CD42b-HIP1), while the activation of GPIIb/IIIa (PAC-1) was increased in UVC-CPPs compared to CPPs. UVC-CPPs demonstrated greater phosph...Continue Reading

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Citations

Feb 7, 2020·International Journal of Molecular Sciences·Katrijn R SixHendrik B Feys
Aug 14, 2019·Journal of Biophotonics·Min LuMei X Wu
Mar 12, 2021·Transfusion·Teresa Jimenez-MarcoEnrique Girona-Llobera
Jun 3, 2021·Journal of Clinical Medicine·Annina CapraruBehrouz Mansouri Taleghani
Oct 13, 2021·Transfusion·Ben WoodLacey Johnson

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