Cryopreservation of whole murine and porcine livers

Rejuvenation Research
Zohar GavishAmir Arav

Abstract

Preservation of vascularized organs, such as the liver, is limited to 24 h before destructive processes disqualify them for transplantation. This narrow window of opportunity prevents the performance of optimal pathogen screening and matching tests and possibly results in the need for retransplantation. Numerous problems are associated with freezing and thawing a whole liver while preserving its viability upon thawing, including complicated geometry, poor heat transfer, release of latent heat, and the difficulty of generating a uniform cooling rate. On the basis of our past success with sheep ovaries, we have now applied our novel freezing technique to a larger solid organ, the liver. Whole rat and pig livers were frozen and thawed using directional solidification apparatus, and viability of these livers was tested by means of integrity and functionality in vitro and in auxiliary liver transplantation. The thawed rat and porcine livers were intact and demonstrated >80% viability. Histology revealed normal architecture. Bile production and blood flow following auxiliary transplantation were normal as well. Our encouraging results in applying this novel cryopreservation technique in rat and pig livers suggest that this method may...Continue Reading

References

Oct 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·J F Carpenter, T N Hansen
Feb 1, 1990·Transplantation·B H PienaarF O Belzer
Sep 9, 1967·Lancet·F O BelzerJ E Dunphy
Mar 1, 1965·Cryobiology·P A Lotke, M F Dolan
Aug 1, 1984·Cryobiology·G M FahyH T Meryman
Jan 1, 1995·Annual Review of Medicine·J H Southard, F O Belzer
Apr 29, 1994·Biochemical and Biophysical Research Communications·B RubinskyC Y Lee
Feb 1, 1996·Biomaterials·J O Karlsson, M Toner
Jan 26, 1999·Annals of the New York Academy of Sciences·P R BarrattJ C Bischof
Nov 10, 2001·Annual Review of Biomedical Engineering·J C Bischof
Apr 12, 2002·The Prostate·Zohar GavishMark Pines
Dec 17, 1957·Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character·A U SMITH
Apr 20, 2004·Cryobiology·Gregory M FahyEric Zendejas
Jun 23, 2005·ASAIO Journal : a Peer-reviewed Journal of the American Society for Artificial Internal Organs·Saravana K Balasubramanian, Robin N Coger
Mar 11, 2008·The Journal of Thoracic and Cardiovascular Surgery·Amir ElamiAmir Arav

❮ Previous
Next ❯

Citations

Dec 7, 2013·Reproduction, Fertility, and Development·Amir Arav, Joseph Saragusty
May 14, 2011·Transfusion Medicine and Hemotherapy : Offizielles Organ Der Deutschen Gesellschaft Fur̈ Transfusionsmedizin Und Immunham̈atologie·Edgardo E GuibertBarry J Fuller
Jan 4, 2013·PloS One·Munekazu NaitoMasahiro Itoh
Jun 3, 2014·European Journal of Medical Research·Zengtao WangZhaowei Zhu
Apr 23, 2014·Nature Reviews. Gastroenterology & Hepatology·Maxim NebrigAndreas Pascher
Jan 26, 2016·Theriogenology·Joseph SaragustyThomas Bernd Hildebrandt
May 25, 2010·Experimental and Toxicologic Pathology : Official Journal of the Gesellschaft Für Toxikologische Pathologie·Hans-Udo KasperVolker Dries
Feb 18, 2010·Reproductive Biomedicine Online·A AravP Patrizio
Aug 1, 2012·Reproduction in Domestic Animals = Zuchthygiene·A Arav, D Natan
Aug 27, 2015·BioResearch Open Access·Gunther Kletetschka, Jolana Hruba
Apr 20, 2017·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·A AravN Shani
Aug 3, 2017·Zygote : the Biology of Gametes and Early Embryos·N J DonfackA P R Rodrigues
Dec 17, 2019·Clinical Medicine Insights. Reproductive Health·Amir Arav, Pasquale Patrizio
Dec 18, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Kankan QinFrancisco M Fernandes
Dec 17, 2020·Frontiers in Medicine·Julie MeneghelG John Morris
Jul 8, 2019·Current Opinion in Biotechnology·Reinier J de VriesKorkut Uygun
Mar 26, 2021·BMC Biology·Sanja BojicJoão Pedro de Magalhães
Apr 9, 2021·Journal of Tissue Engineering and Regenerative Medicine·Bo HeZengtao Wang
Aug 26, 2020·The Journal of Physical Chemistry Letters·Kankan QinFrancisco M Fernandes

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Reproduction in Domestic Animals = Zuchthygiene
Joseph SaragustyAmir Arav
Clinical Obstetrics and Gynecology
Jacques Donnez, Marie-Madeleine Dolmans
© 2022 Meta ULC. All rights reserved