PMID: 6982946Sep 17, 1982Paper

Cryopreserved human cultured T cells as responder cells for the quantitative measurement of interleukin-2: improvement of the assay

Journal of Immunological Methods
M GramatzkiG D Bonnard

Abstract

Large batches of human cultured T cells (CTC) were cryopreserved for later use as responder cells in a proliferation assay for measurement of interleukin (IL)-2 activity. Cryopreservation of CTC could be carried out without considerable loss in viability and cryopreserved and fresh cells showed equally good responses to IL-2. The conditions of IL-2-dependent CTC growth were analyzed, which led to a better evaluation of test results, and had important implications for the calculation of relative IL-2 activity. The repeated use of the same batch of cryopreserved CTC reduced test variability and provided an assay system that allows reliable and reproducible measurement of human IL-2 activity.

Citations

Jun 1, 1990·Veterinary Immunology and Immunopathology·R E TruaxM J Newman
Jul 11, 1983·Nucleic Acids Research·R DevosW Fiers
May 31, 2006·Journal of Peptide Science : an Official Publication of the European Peptide Society·Alex Yuen-Kam TsoiWing-Ping Fong
Jun 1, 1983·Cellular Immunology·R H ButlerM Piantelli
Feb 1, 1984·Clinical Immunology and Immunopathology·G R BurmesterJ R Kalden
Aug 1, 1984·European Journal of Immunology·M GramatzkiJ R Kalden
Nov 1, 1984·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·F B BianchiS Garbisa
Sep 1, 1985·Journal of Virology·B C RodgersJ G Sissons

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