Cryopreserved immature mouse oocytes: a chromosomal and spindle study

Journal of Assisted Reproduction and Genetics
N FrydmanB Maro

Abstract

Cryopreservation of human oocytes might provide an alternative approach to freezing supernumerary embryos obtained during IVF. This process, performed on immature denuded prophase 1 mouse oocytes, was investigated. We first investigated the capacity of frozen, immature, murine oocytes to continue in vitro maturation after thawing. We then evaluated the risk to offspring from chromosomal damage by cytogenetical and cytological (spindle) analysis. Finally, we attempted to determine the reasons for and the stage of maturation failure. A total of 700 immature oocytes was frozen, 629 (90%) were recovered intact after thawing, and 53% extruded the first polar body, versus 74% for the control group. Freezing was not accompanied by an increase in aneuploidy in maturing oocytes (18 and 15% for thawed and control oocytes, respectively). Consequently, the first meiotic division occurred normally, without an increase in nondisjunction. Spindle analysis demonstrated only a few abnormalities (15 and 2% for thawed and control oocytes, respectively) incompatible with further development. Oocytes arrested during in vitro maturation were mainly at the metaphase I stage (64 and 76% for thawed and control oocytes, respectively). Whereas 17% of tha...Continue Reading

Citations

Sep 12, 2007·Human Reproduction Update·Debra A Gook, David H Edgar
Jun 16, 1999·Current Opinion in Obstetrics & Gynecology·E Porcu
Jun 1, 2006·Current Opinion in Obstetrics & Gynecology·Eleonora Porcu, Stefano Venturoli
Apr 10, 2009·Zygote : the Biology of Gametes and Early Embryos·Zengyan WangFangfang He
Aug 21, 2013·Cell Biology International·Woo-In JangNam-Hyung Kim
Jun 22, 2006·Reproductive Toxicology·G AmbrosiniM Moscarini

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