Cryopreserved microencapsulated hepatocytes--transplantation studies in Gunn rats

Transplantation
V DixitG Gitnick

Abstract

Hepatocyte transplantation has been shown to provide significant metabolic support in several animal models of liver diseases. However, for it to be a viable alternative for supplementation of liver function in disease, large quantities of isolated hepatocytes would be necessary. At the present time there are no inexpensive routine methods for cryopreservation of hepatocytes. Existing procedures are cumbersome and require expensive programmable freezers. Hepatocyte cultures are sensitive and easily damaged in handling. By utilizing techniques of microencapsulation and cryopreservation we have attempted to overcome these problems. We have developed a simple, convenient, and inexpensive technique for the long-term storage of hepatocytes. Biological activity of the nonfrozen isolated encapsulated hepatocytes (IEH) and cryopreserved IEH (cIEH) was assessed both in tissue culture and by transplantation in Gunn rats. Significant urea and protein syntheses were detectable during the 10-day culture period even in the 30-day cIEH. Additionally, transplanted IEH and cIEH significantly reduced hyperbilirubinemia in Gunn rats for up to 30 days posttransplantation. Control (empty) microcapsules did not lower serum bilirubin levels. Thus we ...Continue Reading

Citations

Apr 6, 2001·Biotechnology and Bioengineering·A HubelT B Darr
Mar 25, 1994·Biotechnology and Bioengineering·J RozgaT Giorgio
Jan 1, 1995·Transplant International : Official Journal of the European Society for Organ Transplantation·N KokudoM Nozawa
Feb 24, 2001·Journal of Hepatology·T M Chang
Jun 22, 2002·Journal of Hepatology·Shinichiro IkedaYohichi Mochizuki
Jan 29, 2002·Toxicology in Vitro : an International Journal Published in Association with BIBRA·G C McKayM H Grant
Mar 17, 2007·Tissue Engineering·Yingnan WuLilia L Kuleshova
Dec 17, 2009·Tissue Engineering. Part C, Methods·Rita MalpiquePaula M Alves
May 1, 1997·Journal of Orthopaedic Trauma·K J KovalM Chakka
Jan 1, 1994·Transplant International : Official Journal of the European Society for Organ Transplantation·N ArkadopoulosJ Papadimitriou
Aug 3, 1999·Transplant International : Official Journal of the European Society for Organ Transplantation·A OstrowskaB M Bilir
Nov 10, 2001·Annual Review of Biomedical Engineering·E S TzanakakisW S Hu
Apr 14, 2012·International Journal of Biomaterials·Jennifer MumawSteven L Stice
Jan 1, 1995·Journal of Biomaterials Science. Polymer Edition·V Dixit, G Gitnick
Jun 21, 2008·World Journal of Gastroenterology : WJG·Philippe-A LysyEtienne-M Sokal
Sep 26, 2013·Artificial Cells, Nanomedicine, and Biotechnology·Serap DurkutY Murat Elçin
Jan 1, 1995·Artificial Cells, Blood Substitutes, and Immobilization Biotechnology·Z P ChenG Mohr
Dec 12, 2007·Hepatology Research : the Official Journal of the Japan Society of Hepatology·Tomokazu KusanoMitsuo Kusano
Jul 30, 2014·Journal of Tissue Engineering and Regenerative Medicine·Xia XuZhan Feng Cui
Jul 27, 1999·Chemico-biological Interactions·A GuillouzoC Guyomard
Jul 27, 1999·Chemico-biological Interactions·H G KoebeF W Schildberg
May 1, 1995·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·C SeldenH J Hodgson
Jan 1, 1996·Scandinavian Journal of Gastroenterology. Supplement·V Dixit, G Gitnick
Jan 1, 1996·Human & Experimental Toxicology·P Watts, M H Grant
Jan 1, 1995·Scandinavian Journal of Gastroenterology. Supplement·V Dixit
Jul 31, 1999·Artificial Cells, Blood Substitutes, and Immobilization Biotechnology·S Bruni, T M Chang
Jan 19, 2002·Annals of the New York Academy of Sciences·L CanapleB Desvergne

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.