Cryoprotective effects of antifreeze proteins delivered into zebrafish embryos

Cryobiology
S Martínez-PáramoM P Herráez

Abstract

Fish embryo cryopreservation, which is useful in aquaculture or biodiversity conservation, is still far from being achieved. Structural barriers reduce the entrance of cryoprotectants into embryo compartments. Previous studies demonstrated a better ability for freezing in Arctic species which naturally express antifreeze proteins (AFPs). In this study, AFPs were delivered in early zebrafish embryos by incubation in media containing protein. Their cryoprotective effects were then analyzed. Chilling sensitivity was evaluated at 4 degrees C and -10 degrees C. Survival rates significantly increased in embryos incorporating AFPI and kept at -10 degrees C. To analyze their effects on cryopreservation, 5-somite embryos were vitrified. Incorporation of AFPI reduced the percentage of embryos that collapsed at thawing (14.2% of AFPI-treated embryos and 48.9% of controls). Cellular damage caused by vitrification was assessed after thawing by cell dissociation and further analysis of cell survival in culture (SYBR-14/IP labeling). The percentage of viable cells at thawing ranged from 25 to 50%, considered incompatible with embryo development. Cells recovered from frozen-control embryos did not survive in culture. However, the incorporation...Continue Reading

References

Oct 22, 1998·Biology of Reproduction·M HagedornU Pilatus
Apr 20, 2000·The Journal of Biological Chemistry·I ZieglerT McDonaldo
Oct 24, 2000·Journal of Immunological Methods·J Schmidt-MendeB Zhivotovsky
Feb 22, 2001·Annual Review of Physiology·G L FletcherP L Davies
Jun 18, 2002·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·R W R CrevelM J Spurgeon
Aug 24, 2002·Biology of Reproduction·M HagedornB S Pukazhenthi
Nov 26, 2002·Cryobiology·Satoshi KusudaNobuhisa Koide
Sep 10, 2003·Cryobiology·V RoblesM P Herráez
Nov 10, 2004·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·S L LanceM Hagedorn
Jun 7, 2005·Theriogenology·Delgado M ValdezMagosaburo Kasai
May 7, 2008·Cryobiology·S Martínez-PáramoM P Herráez

❮ Previous
Next ❯

Citations

Sep 19, 2009·Zebrafish·Vanesa RoblesM Paz Herráez
Apr 4, 2015·Yonsei Medical Journal·Jung Ryeol LeeSeok Hyun Kim
Sep 5, 2018·Systems Biology in Reproductive Medicine·Peter L Davies, Laurie A Graham
Oct 21, 2009·Applied Microbiology and Biotechnology·Brent C Christner
Mar 22, 2019·Biomolecules·Guizela Huelsz-PrinceKonrad Meister
May 12, 2019·Biomolecules·Vanesa RoblesMarta F Riesco
Dec 16, 2020·Biomolecules·Azadeh EskandariSiti Nurbaya Oslan
Mar 12, 2020·International Journal of Biological Macromolecules·Hong XiangYong Hu
Aug 23, 2021·International Journal of Biological Macromolecules·Abirami BaskaranParli V Bhaskar

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis