Cryostorage of immature and mature human testis tissue to preserve spermatogonial stem cells (SSCs): a systematic review of current experiences toward clinical applications

Stem Cells and Cloning : Advances and Applications
Nima Pourhabibi ZarandiHooman Sadri-Ardekani

Abstract

While the survival rate of children with cancer is increasing, preserving fertility for prepubertal boys is still a challenge. Although intracytoplasmic sperm injection (ICSI) using frozen sperms has revolutionized infertility treatment, it is not applicable for the patients who undergo chemotherapy before puberty since spermatogenesis has not begun. Therefore, preserving spermatogonial stem cells (SSCs) as an experimental option can be provided to prepubertal patients at a risk of damage or loss of their SSCs due to cancer treatments and developmental or genetic disorders. Using frozen SSCs in testicular tissue, successful SSC autotransplantation in mouse and nonhuman primates has shown a promising future for SSC-based cell therapy. Cryopreservation of testicular tissue containing SSCs is the first step to translate SSC-based cell therapy into clinical male infertility treatment, and in the investigation into SSCs, it is very important to evaluate their quantity and functionality during this process. This systematic review summarizes the published data on cryopreservation techniques in human testis tissue for potential utilization in future clinical applications.

Citations

Nov 2, 2019·International Journal of Molecular Sciences·Lihua DongClaus Yding Andersen
Jul 14, 2020·Frontiers in Oncology·Miriam DellinoErica Silvestris
Dec 7, 2018·International Journal of Molecular Sciences·Ali AbuMadighemMahmoud Huleihel
Feb 26, 2020·International Journal of Molecular Sciences·Marion DelessardNathalie Rives
Aug 6, 2020·International Journal of Molecular Sciences·Mahmoud Huleihel, Eitan Lunenfeld
May 23, 2020·Annals of Biomedical Engineering·Justine SaulnierChristine Rondanino

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Methods Mentioned

BETA
biopsy
transmission electron microscopy
biopsies
light microscopy
xenografting
flow cytometry
fluorescence-activated cell sorting

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