Cryosurvival of bovine blastocysts is enhanced by culture with trans-10 cis-12 conjugated linoleic acid (10t,12c CLA)

Animal Reproduction Science
R M PereiraC C Marques

Abstract

An excessive lipid content in embryo cells is a consequence of embryo culture in the presence of serum which is suggested to be responsible for their high susceptibility to cryopreservation. The objective of the present study was to examine the effects of supplementing serum-containing culture media with trans-10 cis-12 conjugated linoleic acid (10t,12c CLA) on embryo lipid accumulation and its subsequent cryopreservation. Abattoir-derived oocytes were matured and fertilized in vitro (IVF=day 0). On day 1, presumptive zygotes (n=3390) were randomly placed in: (I) (MS), granulosa cell monolayer cultured with M199 and 10% serum; (II) (SCLA), granulosa cell monolayer cultured with M199, 10% serum and 100 microM 10t,12c CLA and (III) (SOF), modified synthetic oviduct fluid, where embryo culture proceeded for 8 days. Cleavage rates or D7/D8 embryo quality did not vary among treatments. D7/D8 embryo production rate was significantly (P<0.001) lower in SOF (17.9+/-1.6%) than in groups MS (29.8+/-2.5%) and SCLA (27.8+/-2.0%). After cytoplasmic lipid droplets observation under Nomarski microscopy, classified embryos were the leanest when cultured in SOF, intermediate in SCLA and the fattest in MS (P<0.02). Post-thawing intact blastocyst...Continue Reading

References

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Citations

May 23, 2008·Cell and Tissue Banking·R M Pereira, C C Marques
Dec 8, 2009·Journal of Lipid Research·Christina R FerreiraMarcos N Eberlin
Jul 6, 2010·Prostaglandins & Other Lipid Mediators·Abdulrahman Al DarwichFlorence Guignot
Oct 30, 2013·Molecular Reproduction and Development·Baoyu JiaShien Zhu
Aug 26, 2015·Reproduction in Domestic Animals = Zuchthygiene·C WangJ Li
May 16, 2015·Reproduction in Domestic Animals = Zuchthygiene·Smp TeixeiraF Moreira da Silva
Sep 13, 2014·Zygote : the Biology of Gametes and Early Embryos·Beatriz C S LeãoGisele Z Mingoti
Jun 13, 2015·Reproductive Biology and Endocrinology : RB&E·Joana E MatosRosa M L N Pereira
Dec 22, 2015·Reproduction, Fertility, and Development·Luis BaldocedaClaude Robert
Dec 16, 2017·Zygote : the Biology of Gametes and Early Embryos·Shabnam FayeziMasoud Darabi
Apr 12, 2013·Reproduction, Fertility, and Development·Hanna StinshoffChristine Wrenzycki
May 10, 2013·Reproduction, Fertility, and Development·Monique M GuardieiroRoberto Sartori
Jan 9, 2015·Reproduction, Fertility, and Development·Luis BaldocedaClaude Robert
Oct 11, 2017·Animal Science Journal = Nihon Chikusan Gakkaihō·Ashit Kumar PaulRangsun Parnpai
Jan 1, 2018·Reproduction, Fertility, and Development·Teresa Mogas
Aug 3, 2011·Reproduction in Domestic Animals = Zuchthygiene·J PimentaR M Pereira
Jun 13, 2017·The Journal of Reproduction and Development·Takahito AbeHisataka Iwata
Mar 4, 2011·Reproduction in Domestic Animals = Zuchthygiene·M LapaR M Pereira
Oct 28, 2019·Reproduction in Domestic Animals = Zuchthygiene·Luzia Renata Oliveira DiasMargot Alves Nunes Dode
Sep 8, 2012·Reproduction, Fertility, and Development·Toshikiyo TakahashiNoboru Manabe
Aug 17, 2020·Archives of Biochemistry and Biophysics·S V RannevaN V Surovtsev
Aug 19, 2021·Zygote : the Biology of Gametes and Early Embryos·Clara Slade OliveiraNaiara Zoccal Saraiva
Jul 9, 2014·Journal of Animal Science and Biotechnology·Ribrio Ivan Tavares Pereira BatistaJoão Henrique Moreira Viana

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