PMID: 8584438Jan 1, 1996Paper

Crypt base cells show forskolin-induced Cl- secretion but no cation inward conductance

Pflügers Archiv : European journal of physiology
D EckeR Greger

Abstract

Whole-cell patch-clamp studies in base cells of isolated colonic crypts of rats pretreated with dexamethasone were performed to examine the effects of stimulation by forskolin (10 micromol/l). The experiments were designed in order to distinguish between two postulated effector mechanisms: the activation of a non-selective cation channel and the activation of Cl- channels. As shown in an accompanying report, forskolin depolarizes the membrane voltage (Vm) by some 40-50 mV and enhances the whole-cell membrane conductance (Gm) substantially in these cells. In this report all experiments were performed in the presence of forskolin. A reduction of the bath Na+ concentration from 145 to 2 mmol/l led to a hyperpolarization of Vm by some 20-30 mV. This hyperpolarization occurred very slowly suggesting that the hyperpolarization produced by the low-Na+ solution was caused indirectly and not by a change in the equilibrium potential for Na+, ENa+. A complete kinetic analysis of the effect on voltage of bath Na+ revealed a saturation-type relation with a high apparent affinity for Na+ of around 5-10 mmol/l. A reduction in bath Cl- concentration from 145 to 32 mmol/l caused a depolarization of Vm from -34 +/- 3 to -20 +/- 4 mV (n = 13) in ...Continue Reading

References

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Jan 1, 1996·Pflügers Archiv : European journal of physiology·D EckeR Greger

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Citations

Aug 1, 1996·Pflügers Archiv : European journal of physiology·R Greger
Aug 1, 1996·Pflügers Archiv : European journal of physiology·K G FischerR Greger
Dec 5, 2002·European Journal of Pharmacology·Gisela KuhnErwin Scharrer
Jul 21, 1998·European Journal of Pharmacology·G Schultheiss, M Diener
Jun 9, 2000·Annual Review of Physiology·R Greger
Aug 16, 2002·The Journal of Physiology·Hideki SakaiNoriaki Takeguchi
Oct 22, 2004·European Journal of Pharmacology·Rainer CermakGisela Kuhn
May 20, 2015·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Wiebke C BasseMarkus Bleich
Feb 16, 2005·Annual Review of Physiology·John P Geibel
Jan 1, 1996·Pflügers Archiv : European journal of physiology·D EckeR Greger
May 1, 1996·Pflügers Archiv : European journal of physiology·N BenningR Nitschke
Jan 5, 2002·Physiological Reviews·Karl Kunzelmann, Marcus Mall
Jul 1, 1996·Pflügers Archiv : European journal of physiology·M MallR Greger
Oct 1, 1996·Pflügers Archiv : European journal of physiology·M BleichR Greger
May 1, 1996·Pflügers Archiv : European journal of physiology·M SlawikR Greger
Oct 28, 1998·Physiological Reviews·L P SullivanJ J Grantham
Jul 17, 1999·The American Journal of Physiology·M GrunnetD A Klaerke
Feb 1, 2000·The American Journal of the Medical Sciences·R Greger

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