Crypteins derived from the mouse neuropeptide FF (NPFF)A precursor display NPFF-like effects in nociceptive tests in mice

Peptides
Jolanta KotlinskaJerzy Silberring

Abstract

NPFF precursor, pro-NPFF(A) contains three known bioactive sequences: NPFF (FLFQPQRF-NH(2)), neuropeptide AF (NPAF; AGEGLSSPFWSLAAPQRF-NH(2)) and neuropeptide SF (NPSF; SLAAPQRF-NH(2)). The key-feature of these fragments is their common PQRF-amidated sequence at their C termini. Here, we evaluated the biological activity of two other sequences derived from the mouse NPFF(A) precursor, that does not have PQRF-amidated C-terminus. One peptide was residing between positions 85 and 99 in the mice pro-NPFF(A). This peptide was referred to as neuropeptide SA (NPSA; SAWGSWSKEQLNPQA), assigned due to its flanking amino acids. Another sequence used in the experiments was N-terminal fragment of NPSA, here referred to as neuropeptide SS (NPSS; SAWGSWS). These two peptides, classified as crypteins, were synthesized and tested in the hot-plate and tail immersion tests in mice for their pharmacological activity in morphine-induced antinociception. The effects of both crypteins were compared to NPFF. Our experiments indicated that both crypteins inhibited morphine antinociception and their effects were reversed by RF9, an antagonist of NPFF receptors. These data show that NPSA and NPSS possess NPFF-like anti-opioid activity in these behaviora...Continue Reading

References

Nov 3, 1992·European Journal of Pharmacology·S GicquelJ M Zajac
Oct 28, 1991·Neuroscience Letters·J R LakeD H Malin
Nov 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·H Y YangE Costa
Jun 11, 1993·European Journal of Pharmacology·C GouardèresK Jhamandas
May 21, 1998·European Journal of Pharmacology·M Roumy, J M Zajac
Dec 29, 1999·Brain Research·P PanulaA Pertovaara
Jun 14, 2000·The Journal of Biological Chemistry·N A ElshourbagyH M Sarau
Oct 12, 2000·The Journal of Biological Chemistry·J A BoniniB Borowsky
Mar 1, 1957·British Journal of Pharmacology and Chemotherapy·T J HALEY, W G MCCORMICK
Feb 1, 2005·European Journal of Pharmacology·Isabelle QuelvenJean-marie Zajac
Apr 29, 2005·Current Topics in Medicinal Chemistry·Catherine MollereauJean-Marie Zajac
Sep 1, 2005·Biochemical and Biophysical Research Communications·Anne RoussinJean-Marie Zajac
Jan 13, 2006·Proceedings of the National Academy of Sciences of the United States of America·Frédéric SimoninGuy Simonnet
Jan 31, 2006·Peptides·Hsiu-Ying T Yang, Michael J Iadarola
Oct 20, 2006·Peptides·Feng LiuGeert Wets
Feb 10, 2007·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Jack H JhamandasKim H Harris
Sep 15, 2007·Neuropeptides·Hsiu-Ying T YangMichael J Iadarola

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Citations

Oct 16, 2013·Peptides·Richard J Bodnar
Sep 24, 2014·International Journal of Molecular Sciences·Giuseppe GrassoJerzy Silberring
Jan 7, 2021·Journal of Enzyme Inhibition and Medicinal Chemistry·Dorota WacławczykGiuseppe Grasso

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