Cryptic Diversity, but to What Extent? Discordance Between Single-Locus Species Delimitation Methods Within Mainland Anoles (Squamata: Dactyloidae) of Northern Central America

Frontiers in Genetics
Erich P HofmannJosiah H Townsend

Abstract

Single-locus molecular barcoding is a useful method for identifying overlooked and undescribed biodiversity, providing the groundwork for further systematic study and taxonomic investigation. A variety of methods for delimiting species from barcoding libraries have been developed and applied, allowing for rapid estimates of species diversity in a broad range of taxa. However, tree-based and distance-based analyses can infer different group assignments, potentially over- or underestimating the number of putative species groups. Here, we explore diversity of mainland species of anole lizards from the Chortís Block biogeographical province of northern Central America using a DNA barcoding approach, generating and analyzing cytochrome oxidase subunit I (COI) sequences for over 400 samples assignable to 33 of 38 (86.8%) native and one introduced mainland species. We subsequently tested the effects different models of nucleotide substitution, different species-delimitation algorithms, and reducing our dataset had on species delimitation estimates. We performed of two distance-based (ABGD, RESL) and three tree-based (bPTP, mPTP, GMYC) analyses on both the full dataset and a dataset consisting only of unique halotypes. From 34 nominal ...Continue Reading

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Citations

May 16, 2020·Genome Génome / Conseil National De Recherches Canada·Ben A ParslowMark I Stevens
Nov 8, 2020·BMC Evolutionary Biology·Leona LovrenčićIvana Maguire
Feb 11, 2021·Molecular Ecology Resources·Giulia MagogaMatteo Montagna
Feb 18, 2021·Genome Génome / Conseil National De Recherches Canada·Mario Alejandro MarínCarlos Federico Álvarez
Jul 22, 2021·Molecular Ecology Resources·Aurélien MirallesNicolas Puillandre

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Datasets Mentioned

BETA
GMYC

Methods Mentioned

BETA
electrophoresis
PCR

Software Mentioned

jModelTest
BEAST
mPTP
MEGA7
TreeAnnotator
DAMBE
RAxML
PTP
Tracer
ABGD

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