Abstract
Cryptochrome 1 (CRY1) is a key protein that regulates the feedback loop of circadian clock. The abnormal expression of CRY1 was reported in numerous cancers, and contributed to tumorigenesis and progression. But the underlying mechanism remains undefined. CRY1 overexpression was constructed by lentivirus vector. Gene and protein expression was detected by reverse transcription quantitative polymerase chain reaction and Western blot. Cell proliferation was analyzed by CCK-8 assay. Cell migration ability was analyzed by scratch assay and transwell migration assay. The cAMP concentration was measured by intracellular cAMP assay. Overexpression of CRY1 showed slightly effect on the proliferation and migration of HGC-27 cells. Upon exposure to isoproterenol (ISO), a β-adrenergic receptor agonist, cell proliferation, and migration were inhibited while the cAMP/PKA pathway was activated and ERK1/2 phosphorylation was suppressed. CRY1 overexpression reduced cAMP accumulation, retained ERK1/2 phosphorylation level and alleviated the antiproliferative effect upon exposure to ISO. However, CRY1 overexpression was inoperative on the antiproliferative effect of forskolin (FSK), a direct activator of adenyl cyclase (AC), or 3-isobutyl-1-meth...Continue Reading
References
Nov 12, 1993·Science·J WuT W Sturgill
Nov 12, 1993·Science·S J Cook, F McCormick
Apr 4, 1997·Cell·M R VosslerP J Stork
Aug 28, 1999·The Journal of Biological Chemistry·L L DuganD H Gutmann
Apr 25, 2002·Molecular and Cellular Biology·Amardeep S DhillonWalter Kolch
Jun 21, 2002·Trends in Cell Biology·Philip J S Stork, John M Schmitt
May 16, 2003·Neuropeptides·Céline AlleaumeMichel Philippe
Feb 3, 2009·Haematologica·Eloisa Jantus LewintreJavier García-Conde
Feb 18, 2009·Cell Cycle·Laurence BorgsBrigitte Malgrange
Apr 29, 2010·Cancer Biology & Therapy·Dong ZhangMin Zhang
Sep 21, 2010·Nature Medicine·Eric E ZhangSteve A Kay
Dec 15, 2010·Oncology Research·Mario Pérez-SayánsAbel García-García
Apr 30, 2011·Annual Review of Plant Biology·Inês ChavesMargaret Ahmad
Jul 11, 2012·Proceedings of the National Academy of Sciences of the United States of America·Rajesh NarasimamurthyInder M Verma
Apr 30, 2013·PloS One·Hongyan YuWenlin Huang
May 1, 2013·The Journal of Immunology : Official Journal of the American Association of Immunologists·Ming ShiNing Guo
May 27, 2014·Cell·Ira SchmalenEva Wolf
Jun 7, 2014·Annual Review of Biochemistry·Brian R Crane, Michael W Young
Dec 2, 2014·Immunology Letters·Bing Qin, Yunlong Deng
Mar 2, 2016·Scientific Reports·Falin QuGuoqiang Bao
Feb 16, 2018·Frontiers in Pharmacology·Chiara GiacomelliMaria L Trincavelli
Jul 22, 2018·Journal of Cancer·Lei ZhouMinghai Wang
Oct 18, 2018·Journal of Molecular Endocrinology·Yujiro YamanakaSato Honma
Mar 14, 2019·Biology·Michael H HastingsMarco Brancaccio
Feb 23, 2020·Cells·Elena J KummKerstin Jurk