Cryptochrome 1 from Brassica napus is up-regulated by blue light and controls hypocotyl/stem growth and anthocyanin accumulation

Plant Physiology
Mithu ChatterjeeJitendra P Khurana

Abstract

Cryptochromes are blue/ultraviolet-A light sensing photoreceptors involved in regulating various growth and developmental responses in plants. Investigations on the structure and functions of cryptochromes in plants have been largely confined to Arabidopsis (Arabidopsis thaliana), tomato (Lycopersicon esculentum), and pea (Pisum sativum). We report here the characterization of the cryptochrome 1 gene from Brassica napus (BnCRY1), an oilseed crop, and its functional validation in transgenics. The predicted BnCRY1 protein sequence shows a high degree of sequence identity (94%) to Arabidopsis CRY1. A semiquantitative reverse transcription-polymerase chain reaction and the western-blot analysis revealed that blue light up-regulates its transcript and protein levels in young seedlings. The BnCRY1 promoter harbors conventional light-responsive cis-acting elements, which presumably impart light activation to the GUS (beta-glucuronidase) reporter gene expressed in Arabidopsis. Although the BnCRY1 transcript could be detected in all the tissues examined, its protein was virtually undetectable in mature leaves and the root, indicating a tissue-specific translational control or protein turnover. The antisense-BnCRY1 Brassica transgenic se...Continue Reading

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Citations

Jul 25, 2009·Molecular Biology Reports·Pei XuZheng Qiang Ma
Dec 25, 2008·Proceedings of the National Academy of Sciences of the United States of America·Qingzhu ZhangChentao Lin
Apr 15, 2010·Journal of Experimental Botany·Hui-Ling Liao, Jacqueline K Burns
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Dec 24, 2014·Planta·Yasuko Kadomura-IshikawaSumihare Noji
Jan 3, 2013·American Journal of Botany·Yihai Wang, Kevin M Folta
Sep 28, 2006·Photochemistry and Photobiology·Qing-Hua Li, Hong-Quan Yang
Mar 15, 2020·Annual Review of Plant Biology·Qin Wang, Chentao Lin
Jul 29, 2017·Plant Molecular Biology Reporter·Aliz NovákGábor Galiba
Nov 28, 2020·Planta·Durga Prasad Biswal, Kishore Chandra Sekhar Panigrahi
Dec 2, 2020·Plant Signaling & Behavior·Gongping NieXuefeng Wang
Feb 14, 2021·Plant Physiology and Biochemistry : PPB·Avantika BhaskarJitendra P Khurana

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