Cryptococcal capsular glucuronoxylomannan reduces ischaemia-related neutrophil influx

European Journal of Clinical Investigation
Pauline M EllerbroekFrank E J Coenjaerts

Abstract

The capsular polysaccharide glucuronoxylomannan (GXM) of Cryptococcus neoformans interferes with the chemotaxis and transendothelial migration of neutrophils. Intravenous administration of purified GXM has been shown to reduce the influx of inflammatory cells in an animal model of bacterial infection. Here we show that isolated GXM can also interfere with neutrophil migration in a model of inflammation not related to infection. We assessed the effects of intravenous GXM on neutrophil infiltration in a rat model of myocardial ischaemia, where neutrophil infiltration has been shown to contribute to postischaemic reperfusion injury. Rats were subjected to coronary artery ligation followed by a 3-h reperfusion period. Myeloperoxidase-activity was measured in the ischaemic tissues as a marker of neutrophil infiltration. Intravenous administration of GXM markedly reduced the influx of neutrophils in the ischaemic myocardium as measured by a 65% reduction of tissue MPO activity. This reduction of MPO activity was clearly correlated to the serum concentration of GXM. As complement activation by GXM was minimal at the doses applied in vivo, it is unlikely that generation of chemotactic C5a in the circulation by GXM caused the observed r...Continue Reading

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Citations

May 31, 2011·Journal of Toxicology and Environmental Health. Part a·Edilson R Alves-FilhoJane L Dos Santos
Dec 9, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Pauline M EllerbroekFrank E J Coenjaerts

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