Cryptococcus neoformans can form titan-like cells in vitro in response to multiple signals

PLoS Pathogens
Nuria Trevijano-ContadorOscar Zaragoza

Abstract

Cryptococcus neoformans is an encapsulated pathogenic yeast that can change the size of the cells during infection. In particular, this process can occur by enlarging the size of the capsule without modifying the size of the cell body, or by increasing the diameter of the cell body, which is normally accompanied by an increase of the capsule too. This last process leads to the formation of cells of an abnormal enlarged size denominated titan cells. Previous works characterized titan cell formation during pulmonary infection but research on this topic has been hampered due to the difficulty to obtain them in vitro. In this work, we describe in vitro conditions (low nutrient, serum supplemented medium at neutral pH) that promote the transition from regular to titan-like cells. Moreover, addition of azide and static incubation of the cultures in a CO2 enriched atmosphere favored cellular enlargement. This transition occurred at low cell densities, suggesting that the process was regulated by quorum sensing molecules and it was independent of the cryptococcal serotype/species. Transition to titan-like cell was impaired by pharmacological inhibition of PKC signaling pathway. Analysis of the gene expression profile during the transit...Continue Reading

References

Dec 1, 1985·Journal of Clinical Microbiology·G L LoveD L Greer
Aug 1, 1985·The Journal of Clinical Investigation·D L GrangerD T Durack
Oct 1, 1970·Journal of Bacteriology·K H NealsonJ W Hastings
Jan 1, 1993·The Journal of Infectious Diseases·S E VartivarianE S Jacobson
Mar 1, 1993·Journal of Bacteriology·D L ToffalettiJ R Perfect
Nov 25, 2000·Medical Mycology·M S LazeraB Wanke
Mar 7, 2002·Proceedings of the National Academy of Sciences of the United States of America·Stephanie C Tucker, Arturo Casadevall
Feb 22, 2003·Infection and Immunity·Mairi C NoverrGary B Huffnagle
Aug 23, 2003·Infection and Immunity·Kirsten NielsenJoseph Heitman
Mar 12, 2004·The Journal of Biological Chemistry·Lena J HeungMaurizio Del Poeta
Apr 23, 2004·Biological Procedures Online·Oscar Zaragoza, Arturo Casadevall
Dec 2, 2004·Proceedings of the National Academy of Sciences of the United States of America·S E KiddW Meyer
Feb 22, 2005·Molecular Microbiology·Tianshun LianJames W Kronstad
Feb 25, 2005·Molecular Biology of the Cell·Yong-Sun BahnJoseph Heitman
Oct 11, 2005·Annual Review of Cell and Developmental Biology·Christopher M Waters, Bonnie L Bassler
Nov 24, 2005·Current Biology : CB·Torsten KlengelFritz A Mühlschlegel
Jan 10, 2006·Eukaryotic Cell·Estelle Geweiss MogensenFritz A Mühlschlegel
Feb 8, 2007·PLoS Medicine·Françoise DromerUNKNOWN French Cryptococcosis Study Group
May 12, 2009·Advances in Applied Microbiology·Oscar ZaragozaArturo Casadevall
Aug 14, 2009·Journal of the Royal Society, Interface·Steve Atkinson, Paul Williams
Jun 30, 2010·PLoS Pathogens·Oscar ZaragozaArturo Casadevall
Jun 30, 2010·PLoS Pathogens·Laura H OkagakiKirsten Nielsen

❮ Previous
Next ❯

Citations

May 24, 2019·Virulence·Oscar Zaragoza
May 30, 2019·Microbiology·Maria MaryamCarolina Coelho
Jan 29, 2020·Infection and Immunity·Nuria Trevijano-ContadorLiise-Anne Pirofski
Feb 28, 2020·Journal of Fungi·Tyler G NormileMaurizio Del Poeta
Jul 6, 2019·Frontiers in Cellular and Infection Microbiology·Mélissa Caza, James W Kronstad
Nov 11, 2019·Genes·Asiya Gusa, Sue Jinks-Robertson
Aug 9, 2020·F1000Research·Shelby J PriestJoseph Heitman
Dec 28, 2018·Journal of Fungi·Nuria Trevijano-Contador, Oscar Zaragoza
Apr 17, 2019·Journal of Fungi·Ambre F ChapuisDonna M MacCallum
May 28, 2019·Current Tropical Medicine Reports·N Trevijano-Contador, L Pirofski
Jan 30, 2020·Frontiers in Microbiology·Rocio Garcia-RubioNuria Trevijano-Contador
Jun 6, 2020·Virulence·Mariusz DylągLukasz Kozubowski
Feb 29, 2020·Current Biology : CB·Youbao ZhaoXiaorong Lin
Nov 26, 2019·Current Opinion in Microbiology·Hanna ZafarKirsten Nielsen
Jan 16, 2021·Scientific Reports·Elias Barbosa da Silva-JuniorCelio Geraldo Freire-de-Lima
Oct 31, 2020·Pathogens·Elizabeth A GaylordTamara L Doering
Mar 19, 2021·Frontiers in Microbiology·Glauber R de S AraújoSusana Frases
Jan 28, 2021·FEMS Microbiology Reviews·Aleeza C Gerstein, Nathaniel P Sharp
Apr 7, 2021·G3 : Genes - Genomes - Genetics·Corinne MaufraisGuilhem Janbon
Jul 1, 2019·Microbes and Infection·Guilhem JanbonChristophe d'Enfert
Aug 11, 2021·Therapeutic Delivery·Thiago Aparecido da Silva
Sep 17, 2021·Scientific Reports·Israel Diniz-LimaCelio Geraldo Freire-de-Lima
Aug 5, 2021·Annual Review of Microbiology·Xiuyun TianLinqi Wang

❮ Previous
Next ❯

Datasets Mentioned

BETA
GSE111400

Methods Mentioned

BETA
flow cytometry
RNAseq
protein folding
dissection
PCR
reverse-phase chromatography
optical microscopy
confocal microscopy
FCS
FACS

Software Mentioned

ImageJ
FastQ
Adobe Photoshop
Excel
Annotated Probe Report
SeqMonk
Blastp
FlowJo
Leica
Leica Mycrosystems

Related Concepts

Related Feeds

Cell Checkpoints & Regulators

Cell cycle checkpoints are a series of complex checkpoint mechanisms that detect DNA abnormalities and ensure that DNA replication and repair are complete before cell division. They are primarily regulated by cyclins, cyclin-dependent kinases, and the anaphase-promoting complex/cyclosome. Here is the latest research.

Cell Cycle Pathways

Cell cycle is a complex process regulated by several signal transduction pathways and enzymes. Here is the latest research on regulation of cell cycle and cell cycle pathways.

Advanced Imaging of Cellular Signaling

Cell signaling is a vital mechanism for communication within cells and outside with the environment. Several different signaling pathways have been found and advanced imaging techniques are being developed to visualize the molecules involved in these signaling pathways. Find the latest research in advanced imaging of cellular signaling here.

Cardiomegaly

Cardiomegaly, known as an enlarged heart, is a multifactorial disease with different pathophysiological mechanisms. Hypertension, pregnancy, exercise-induced and idiopathic causes are some mechanisms of cardiomegaly. Discover the latest research of cardiomegaly here.

Related Papers

Biological Procedures Online
Oscar Zaragoza, Arturo Casadevall
PLoS Pathogens
Oscar ZaragozaArturo Casadevall
Zentralblatt Für Bakteriologie, Parasitenkunde, Infektionskrankheiten Und Hygiene. Erste Abteilung Originale. Reihe A: Medizinische Mikrobiologie Und Parasitologie
F Staib
© 2022 Meta ULC. All rights reserved