Cryptolepine, the Main Alkaloid of the Antimalarial Cryptolepis sanguinolenta (Lindl.) Schlechter, Induces Malformations in Zebrafish Embryos

Biochemistry Research International
Kwesi Boadu MensahCharles Ansah

Abstract

Previous studies on cryptolepine, the antimalarial and cytotoxic alkaloid of Cryptolepis sanguinolenta, showed that it preferentially accumulates in rapidly proliferating cells and melanin-containing tissues. Subsequently, we demonstrated that cryptolepine was toxic to murine embryos in vivo but no signs of teratogenicity. in vivo developmental studies can be confounded by maternal effects. Here, we hypothesized that cryptolepine-induced embryo toxicity occurs at least partly through direct inhibition of embryogenesis rather than indirectly through the induction of maternal toxicity. To determine the effects of cryptolepine on developing zebrafish embryos ex vivo. Healthy synchronized zebrafish eggs were treated with cryptolepine (10-1 - 5 × 102 μM), benzyl penicillin (6 - 6 × 102 μM), or mercury chloride (3.7 × 10-1 - 3.7 × 101 nM) from 6 to 72 hours postfertilization. Developing embryos were assessed at 24, 48, 72, and 96 hours under microscope for lethality, hatching rate, and malformation. LC50 for cryptolepine in the study was found to be 260 ± 0.174 μM. Cryptolepine induced dose- and time-dependent mortality from the 24 to 96 hours postfertilization. Lower cryptolepine concentration (<100 μM) caused mortality, approximate...Continue Reading

References

Nov 1, 1966·Acta paediatrica Scandinavica·K S Larsson, M Eriksson
Jul 1, 1995·Developmental Dynamics : an Official Publication of the American Association of Anatomists·C B KimmelT F Schilling
Dec 4, 2001·Nature Structural Biology·John N LisgartenJuan Aymami
Nov 21, 2002·Toxicological Sciences : an Official Journal of the Society of Toxicology·Charles Ansah, Nigel J Gooderham
Mar 3, 2006·Toxicological Sciences : an Official Journal of the Society of Toxicology·Huijun Zhu, Nigel J Gooderham
Jul 24, 2007·Bioorganic & Medicinal Chemistry·Scott SevilleColin W Wright
Mar 17, 2009·Phytotherapy Research : PTR·Olumayokun A OlajideColin W Wright
Apr 5, 2011·European Journal of Medicinal Chemistry·Tryphon K MazuSeth Y Ablordeppey
Oct 19, 2013·Toxicological Sciences : an Official Journal of the Society of Toxicology·Lisa TruongRobert L Tanguay
Feb 18, 2016·Malaria Journal·Arnold D ForkuoMichael F Ofori
Dec 29, 2017·Malaria Journal·Arnold Donkor ForkuoColin W Wright
Jan 1, 2017·Advances in Pharmacological Sciences·Newman OsafoOduro Kofi Yeboah
Mar 22, 2019·Drug and Chemical Toxicology·Qiang YeLi Guo

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Citations

Nov 19, 2020·Drug Design, Development and Therapy·Abdrrahman Shemsu SururKaleab Asres

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Methods Mentioned

BETA
column chromatography

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Image J

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