PMID: 1200738Oct 27, 1975Paper

Cryptomonad biliprotein: phycocyanin-645 from a Chroomonas species

Archives of Microbiology
E Mörschel, W Wehrmeyer

Abstract

The properties of phycocyanin-645 from the fresh water cryptomonad Chroomonas spec. were investigated after the pigment was isolated and purified by a combination of differential ammonium sulphate fractionation, gel filtration chromatography and ammonium sulphate gradient elution. Phycocyanin-645 is characterized by absorption maxima at 645 nm, 584 nm, 369 nm, 275 nm and shoulders at 340 nm and 620 nm. The CD spectrum has a negative maximum at 645 nm and a positive maximum at 584 nm with a shoulder at 610 nm. The fluorescence emission spectrum is asymmetrical and shows a maximum at 660 nm and a shoulder at approximately 715 nm. The molecular weight of the native phycocyanin-645, estimated by gel filtration, is 45000 for all multiple pigment forms below. Phycocyanin-645 is heterogeneous as revealed by isoelectric focusing with pIs at 7.03, 6.17, 5.75, 5.25 and 4.88, respectively, the main bands lying at pI 7.03 and pI 6.17. This was confirmed by polyacrylamide gel electrophoresis; five pigment compoents differing in mobility were found. We propose the term "multiple pigment forms" for these five phycocyanin-645 modifications. Calibrated SDS gel electrophoresis shows phycocyanin-645 to consist of three subunits, two light chains ...Continue Reading

References

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Citations

Apr 1, 1989·Photosynthesis Research·J Biggins, D Bruce
May 1, 1996·Photosynthesis Research·G J WedemayerA N Glazer
Oct 11, 1976·Archives of Microbiology·D A BryantF A Eiserling
May 13, 1977·Archives of Microbiology·E Mörschel, W Wehrmeyer
Mar 27, 1992·Biochimica Et Biophysica Acta·J Godovac-ZimmermannR G Hiller
Jan 1, 1986·Photochemistry and Photobiology·D Guard-Friar, R MacColl
Aug 17, 2011·Biophysical Journal·Alessandro MarinRienk van Grondelle
Nov 1, 1976·Archives of Biochemistry and Biophysics·R MacCollO Gibbons
Nov 1, 1995·Photosynthesis Research·A N Glazer, G J Wedemayer

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