Cryptosporidiosis outbreak in a child day-care center caused by an unusual Cryptosporidium hominis subtype

Enfermedades infecciosas y microbiología clínica
Pilar GoñiJosé Gutiérrez-Fernández

Abstract

This work describes the genetic characterization of Cryptosporidium and Giardia involved in an outbreak in a nursery school in Granada, Spain, that affected seven children under the age of 4. Nucleic acids were extracted from the seven stool samples positive to Cryptosporidium or Giardia by microscopy and/or immunochromatography. The species and subtypes of Cryptosporidium were identified by PCR-RFLP and PCR of the SSUrRNA and gp60 genes, respectively. The assemblages of Giardia duodenalis isolates were characterized by PCR of the tpi gene. PCR products were sequenced and analyzed. All of the isolates were positive for Cryptosporidium hominis. Five of them belonged to subtype IaA11R2, one to subtype IbA10G2R2, and the other could not be identified. Three of these samples were positive for G. duodenalis by PCR, two belonging to the assemblage A, and the other one to assemblage B. This is the first report of Cryptosporidium hominis subtype IaA11R2 as a cause of an outbreak in Europe where subtype IbA10G2R2 is the most frequently identified. In the case of Giardia, an outbreak could not be confirmed because of the low number of positive samples and the low genetic variability of the amplified fragments for assemblage A of tpi gene...Continue Reading

References

Jun 7, 2003·Journal of Clinical Microbiology·Margarida AlvesFrancisco Antunes
Jan 14, 2004·Emerging Infectious Diseases·Irshad M SulaimanLihua Xiao
Jun 16, 2005·Journal of Clinical Microbiology·Irshad M SulaimanLihua Xiao
Mar 27, 2007·Parasitology International·María Teresa LlorenteRafael Gómez-Lus
Aug 4, 2007·The Journal of Infectious Diseases·Vitaliano A CamaLihua Xiao
Mar 8, 2008·Emerging Infectious Diseases·Rachel M ChalmersPaul Hunter
Oct 2, 2008·Emerging Infectious Diseases·Vitaliano A CamaLihua Xiao
Nov 26, 2008·Experimental Parasitology·Michelle L PowerSally Williamson
Mar 24, 2009·Experimental Parasitology·Ronald Fayer
Apr 11, 2009·Experimental Parasitology·Lihua Xiao
Aug 8, 2009·Veterinary Parasitology·Judit Plutzer, Panagiotis Karanis
Mar 29, 2011·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Yvonne A L LimRobin B Gasser
Apr 8, 2011·Journal of Clinical Microbiology·Kirthi G HiraHonorine D Ward
Jun 26, 2012·Enfermedades infecciosas y microbiología clínica·Joanna CieloszykAntonio Clavel
Jan 31, 2014·Journal of Clinical Microbiology·Yaoyu FengLihua Xiao

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Citations

Nov 1, 2015·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Aida de LucioDavid Carmena
May 14, 2016·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·S M Cacciò, R M Chalmers
Nov 17, 2017·Clinical Microbiology Reviews·Lynne S GarciaGovinda Visvesvara
Jul 1, 2020·Parasitology Research·Ana Luz Galvan-DiazGisela Garcia-Montoya

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